大鼠脑内白细胞介素-1Ⅰ型受体在致痫活动中的表达变化

CHANGES OF TYPE Ⅰ INTERLEUKIN-1 RECEPTOR EXPRESSION IN BRAIN OF EPILEPTIC RATS

为了探讨白细胞介素 -1I型受体 ( IL-1RI)在癫痫发病中的作用 ,本实验采用免疫组织化学方法观察了致痫大鼠行为改变与脑内 IL-1RI表达变化。结果发现 ,侧脑室注射白细胞介素 -1β( IL-1β)后再注射阈下剂量谷氨酸钠 ,可导致动物痫样发作 ,其大脑皮质及海马锥体细胞层 IL -1RI免疫反应阳性细胞数量较对照组明显增多 ,免疫反应增强 ;如先注射白细胞介素 1受体拮抗剂 ( IL-1ra)、再注射 IL-1β和阈下剂量谷氨酸钠 ,则动物无痫样发作 ,且大脑皮质及海马锥体细胞层 IL-1RI免疫反应阳性细胞数量较注射 IL -1β和阈下剂量谷氨酸钠组减少 ,免疫反应着色减弱。结果提示 ,IL -1β有明显促进谷氨酸钠致痫的作用 ,IL-1RI可能参与致痫过程 ,IL-1ra具有抗痫效应。

In order to investigate the effect of IL-1 RI in epilepsy, the expression changes of IL-1 RI in brain of epileptic rats were studied by immunohistochemical staining, and the behavior changes were observed.The results showed that there was epileptic seizure after injecting IL-1β and subthreshold dose of sodium glutamate into the lateral ventricle of rats, and the numbers of IL-1 RI immunoreactive(IR) positive cells in hippocampus and cerebral cortex were significantly increased compared with the control group. Whenever the IL-1ra was injected into the lateral ventricle before IL-1β and subthreshold dose of sodium glutamate, seizure was restrained and the numbers of IL-1 RI-IR positive cells in hippocampus and cerebral cortex were significantly decreased compared with the previous group. These data suggest that IL-1β can promote the effect of sodium glutamate to induce the epileptic seizure, IL-1 RI might play an important role in epileptogenesis, while IL-1ra can restrain the seizure.