D-和L-肉碱对映体的毛细管电泳分离研究(英文)

Enantiomeric separating study of D-and L-carnitine with capillary zone electrophoresis

提出了用毛细管区带电泳分离和检测肉碱对映体的方法。肉碱消旋体用4-溴苯甲酰甲基溴衍生化,并经选择性手性试剂——磺化β-环糊精和手性冠醚[(S,S)-1,7-bis(4-苯基-5-羟基-2-氧-3-氮苄基)-1,7-二氮杂-12-冠-4]协同作用后,可得到良好分离,并用紫外检测器检测,两异构体的检测下限约为80ng/mL。

A new capillary zone electrophoretic method has been developed for the enantiomeric separation and quantification of enantiomers of carnitine. D-and L-carnitine were derivatized with 4'-bromophenacyl bromide producing a derivatization that can be detected by UV absorbance detection. The separation was performedby using a selective chiral containing sulfated-β-cyclodextrin and (S, S)-1 ,7-bis(4-benzyl-5-hydroxy-2-oxo-3-azapentyl)-1,7-diaza-12-crown-4. Acetonitrile was used as electroosmotic modifier and separation was carried out in an uncoated capillary. Under the optimal conditions D-and L-camitine were separated completely and the limits of detection for both isomers were about 80 ng/mL.