麻疯树营养器官和种子的蛋白质组成及对水分和温度胁迫的反应

Proteins in vegetative organs and seeds of Jatropha curcas L.and those induced by water and temperature stress

用不同溶剂提取麻疯树根、茎、叶及种子的蛋白质,结果表明总蛋白以种子中含量最高,而营养器官中蛋白质主要集中在叶。萌发种子中蛋白质组分变化显著,而贮藏种子中蛋白质组分无太大变化。当年生种子、贮藏种子、茎和叶中所含蛋白质,以碱提组分含量最高;萌动种子和根中的蛋白质以水提组分含量最高。种子中抗癌蛋白质(28KD)、抗真菌蛋白质(22KD、21KD和 20KD三亚基组成 64KD)在碱提组分中含量最高。用 30％PEG-6000处理麻疯树苗,发现在叶中诱导产生55KD、46KD、36KD、30KD、19KD、16KD和13KD多肽,在茎中产生33KD、30KD和23KD多肽,同时42KD和17KD多肽的含量减少。用4℃和51℃处理麻疯树苗,发现叶中54KD和24KD多肽在4℃和51℃均被诱导增加,44KD和36KD多肽在4℃和51℃均减少;茎中4℃和51℃诱导产生52KD、44KD和14KD多肽,诱导32KD、30KD和14KD多肽的增加。水分胁迫和温度胁迫在麻疯树根中未引起蛋白质组成的明显变化。

Proteins were obtained with different solutions from roots, stems, leaves and seeds of Jatropha cur- cas L. The seeds gave the highest percent of total proteins. Of the vegetative organs, the proteins were mainly dis- tributed in leaves. Proteins extracted from germinated seeds had a noticeable decrease compared with current growth seeds. Little difference of protein extracted from stored seeds were found. Among the proteins extracted with four solutions, the proteins derived from stems, leaves and current growth seeds extracted with NaOH solution at the highest percent, while proteins from germinated seeds and roots extracted with H_2O at highest rate. The anti - cancer and antifungi proteins (28KD and 64KD) were extracted by NaOH solution at the highest percent. The pep- tides of 55KD, 46KD, 36KD, 30KD, 19KD, 16KD and 13KD were induced from seedling leaves with 30% poly- ethyenes glycol (PEG -- 6000) -- water stress. The induced peptides of 33KD, 30KD and 23KD were determined and the content decrease of 42KD and 17KD peptides were occurred in stems with 30% polyethyenes glycol (PEG - 6000) -- water stress. The peptides of 54KD, 24KD were induced and 44KD, 36KD were reduced with 4℃ and 51℃ temperature stress. Specific induced peptides of 52KD,44KD and 14KD were produced with 4℃ and those of 32KD, 30KD, 14KD with 51℃ were found. No obvious difference was observed with water stress and temperature stress in roots.