双龙方与自体骨髓单个核细胞经心导管移植对中国小型猪心肌梗死的影响

The effects of Shuanglongfang therapy plus bone marrow mononuclear cells autotransplantation on myocardial infarction in swines

目的:研究中药双龙方与自体骨髓单个核细胞经心导管移植对中国小型猪心肌梗死的影响。方法:利用心导管技术建立中国小型猪冠状动脉栓塞心肌梗死模型,建立经心导管冠状动脉内骨髓单个核细胞移植的新方法,通过心电图、定量组织学、超声心动图、心肌酶及生化检查、心肌小血管密度、梗死区内骨髓单个核细胞观察等综合评价双龙方、自体骨髓单个核细胞经心导管移植及两者合用的疗效。结果:双龙方与自体骨髓单个核细胞经心导管移植可减小心肌梗死范围,超声心动图检查显示心功能明显改善,并可增加心肌梗死区小血管密度,对心肌酶及生化指标亦有不同程度改善作用。自体骨髓单个核细胞移植及合用双龙方后均有助于心肌侧枝循环的建立,心肌供血明显改善。自体骨髓单个核细胞可在移植区内存活,并部分分化为心肌细胞及心肌小血管,补充了心肌细胞的数量。结论:双龙方与自体骨髓单个核细胞移植对中国小型猪心肌梗死有明确的治疗作用。

Objective: To study the effects of shuanglongfang therapy plus bone marrow mononuclear cells autotransplantation on myocardial infarction in swines. Methods:The myocardial infarction model in swines was established by embolizing coronary artery with cardiac catheter and bone marrow mononuclear cells were transplanted into coronary artery through the cardiac catheter. The effects of both shuanglongfang therapy plus bone marrow mononuclear autotransplantation on myocardial infarction in swines were evaluated by cardiogram, histomorphometry, echocardiogram, myocardial enzyme, biochemistry, mini-vessel density of myocardium and observation of bone marrow mononuclear cells in infarction area. Results: The myocardial infarction area in swines could be reduced by shuan-glonfang therapy plus bone mononuclear cells autotransplantation. Echocardiogram indicated that cardiac function was significantly improved and mini-vessel density in infarction area was increased. The myocardial enzyme and biochemical parameters were also improved differently. Bone marrow mononuclear cells autotransplantation in combination of shuanglongfang therapy was helpful to establish collateral circulation and improve blood supply for myocardium. The bone marrow mononuclear cells transplanted can keep alive and part of them was differentiated into myocardial cells and mini-vessel. Conclusion : Shuanglongfang therapy plus bone marrow mononuclear cells transplantation are obviously effective in treatment of myocardial infarction in swines.