彩色多普勒超声对胎鼠小肠上皮细胞凋亡影响的实验研究

The relationship of diagnostic CDFI and apoptosis of fetal rat's intestinal epithelial cell

目的 :探讨彩色多普勒超声对胎鼠小肠粘膜上皮细胞是否构成影响。方法 :2 4只孕 14天SD大鼠按彩超辐照时间不同随机等分为四组 :Ⅰ组 (对照组 ) ,Ⅱ组 (照射 10min组 ) ,Ⅲ组 (照射 2 0min组 ) ,Ⅳ组 (照射 30min组 )。仪器采用Accuson公司Sequeoia 5 12型彩色电脑声像仪 ,照射条件 :4V1探头 ,二维频率H3.0MHz ,彩色频率 3.0MHz,Tis=1.8,Micd =1.6。照射后 2 4h取胎鼠小肠标本 ,HE染色光镜观察 ,TUNEL法检测凋亡细胞 ,透射电镜观察超微结构的变化。结果 :各组光镜下观察均未见异常 ,TUNEL法检测结果 ,Ⅰ组及Ⅱ组胎鼠小肠上皮可见散在凋亡细胞 ,Ⅲ组及Ⅳ组凋亡细胞明显增多。Ⅲ组及Ⅳ组荧光强度与Ⅰ组有显著差异 (ⅢvsIP <0 .0 5 ,IVvsIP <0 .0 1) ,荧光强度与照射时间呈正相关 (P <0 0 1) ,(r=0 .5 71)。Ⅳ组标本电镜检查发现核染色质浓缩边集 ,内质网及线粒体肿胀等超微结构变化。结论 :彩色多普勒超声照射孕鼠 2 0min以上可引起胎鼠小肠粘膜上皮细胞凋亡。

Objective:To study if diagnostic CDFI can induce fetal rat's intestinal epithelial cell apoptosis.Methods:Twenty-four female rats that have been pregnant for 14 days were divided into four groups averagely and randomly,control group(Ⅰ group) was sham-irradiation,Ⅱ-Ⅳ groups were irradiated by diagnostic CDFI (Sequeoia-512,4V1,H3.0MHz,CD=3.0MHz,Tis=1.8,MIcd=1.6) for 10 minutes,20 minutes and 30 minutes respectively,fetal rat's intestines of four groups were removed at 24 hours after irradiation,HE stain was done for microscope observing,intestinal epithelial apoptosis cells were detected with TUNEL(in situs terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) and trasmission electron micrsocope.Results:There were no abnormal changes among Ⅰ-Ⅳ group under microscope,but apoptosis cells increased remarkably in Ⅲ and Ⅳ group(Ⅲ vs I P<0.05,IV vs I P<0.01),apoptotic changes were observed in Ⅳ group using transmission electron mecroscope,which showed margination and condensation of the nuclear chromatin,etc.Conclusion:Continuous irradiation beyond 20 minutes by diagnostic CDFI may result in fetal rat's intestinal epithelial cell apoptosis.