创面修复中C-myc原癌基因表达变化的观察

Observation of C-myc proto-oncogene expression during the clinical wound healing

目的:观测创面修复中C-myc原癌基因的表达变化,探讨C-myc原癌基因表达变化在创面修复中的作用。方法:应用原位杂交方法对临床患者断层供皮区创面愈合中C-myc原癌基因表达变化进行了动态观察,并进行了半定量分析。结果:临床创面愈合中C-myc原癌基因有明显的规律性的表达变化,伤后4d时,C-myc原癌基因在创面内有明显的表达,表达强度+～。伤后10d时,C-myc原癌基因在创面内表达较伤后4d时增强,除分布在创面浅层的成纤维细胞胞浆和创缘上皮基底细胞胞浆以外,尚可见分布在血管内皮细胞和皮肤附属上皮细胞中,创面的中层和深层也可见有表达,多较密集分布,表达强度～。伤后16d时,C-myc原癌基因在创面内表达的强度较伤后10d时明显减弱,表达强度接近伤后4d时的水平,表达强度+。结论:C-myc原癌基因表达与临床创面修复的过程有明显相关,C-myc原癌基因表达变化参与了临床创面修复过程,合理调控其表达变化有助于临床创面修复。

A IM:To detect the changes of C-myc proto-oncogene expression and to ex-plore its roles during the clinical wound healing.METHODS:Dynamic observation and se miquanti tative analysis were ex-amined in 22patients during the wound healin g of splitting skin donor using the in situ hybridization method.RESULTS:In cl inical wound healing,changes o f C-myc proto-oncogene ex-pression were obser ved obviously.C-myc proto-oncogene expressed more ob-viously at 10day(str ength of-)than at 4day(strength of +-)after wound.At 10day,C-m yc expression was observed in vascular en-dothelial cells and dermal epithelial cells,in middle and deep layer of wound,and in also superficial layer of fibr oblast cytoplasm and basal ce lls on the wound margins,most of whose distributi on was denseness.C-myc pro-to-oncogene expression at 16day(strength of + )was decreased compared with that at 10day,and was close to th at at 4day.C-myc proto-oncogene expression wa s obviously correlated with the clinical wound healing.The changes of its expression take part i n the clinica l wound healing process.It i s helpful for the clinical wound healing to reason ably control the