硝酸镧染毒对小鼠骨髓细胞微核率的影响

Effects of Exposure to Lathanum Nitrate on Micronucleus Frequency of Bone Marrow Cells Among Mice

目的研究硝酸镧染毒对小鼠骨髓细胞微核率的影响。方法将21日龄昆明种小鼠随机分为9组,每组12只,分别腹部皮下注射不同剂量的硝酸镧溶液(10、50、100、200、300、400、500mg/kg),阴性对照组给予蒸馏水,阳性对照组给予环磷酰胺,每天1次,连续7d,观察小鼠骨髓细胞微核率及小鼠体重。结果300、400、500mg/kg组小鼠增重低于阴性对照组,差异有显著性(P<0.05)。100、200、300、400、500mg/kg组小鼠骨髓细胞微核率高于阴性对照组(P<0.05)。随染毒剂量增加,小鼠骨髓细胞微核率呈上升趋势(r=0.9223P<0.05)。结论硝酸镧具有一定遗传毒性。

Objective To study the effects of exposure to lanthanum nitrate(LaNO3)on the micronucleus frequency of bone marrow cells among mice. Methods Kunming mice aged 21 days were randomly divided into 9 groups, 12 mice per group. The 9 groups included 7 LaNO3-treated groups at abdomenal area subcutaneously injected with LaNO3 solution at different doses of 10, 50, 100, 200, 300, 400, 500 mg/kg respectively, negative control group treated with distilled water and positive control group treated with cyclophosphamide. The treatment was performed once a day, continuously for 7 days. After 7-day treatment, the micronucleus frequency of bone marrow cells and body weight were measured among mice. Results The increases of body weights of mice in 300, 400 and 500 mg/kg LaNO3 exposure groups were significantly lower than those of negative control group(P<0.05). The micronucleus frequencies of bone marrow cells of mice in 100, 200, 300, 400 and 500 mg/kg groups were significantly higher than those of negative control groups(P<0.05). With the increase of the exposure doses of LaNO3, the micronucleus frequencies of bone marrow cells in mice increased gradually among mice (r=0.922 3, P<0.05). Conclusion LaNO3 presented a certain genotoxicity.