原发性高血压并发脑梗死患者血管紧张素转换酶和血管紧张素受体基因多态性的研究

The polymorphism of angiotensin converting enzyme gene and angiotensin receptor gene in patients with essential hypertension complicated by brain infarction

目的 :探讨血管紧张素转换酶 (ACE)基因及血管紧张素Ⅱ 1型受体 (AT1R)基因A116 6 /C多态性与原发性高血压 (EH)及其并发脑梗死的关系。方法 :应用聚合酶链反应及PCR加酶解方法检测 15 0例健康者 (对照组 )及 15 2例EH无并发症患者 (EH组 )和 80例EH并发脑梗死患者 (EH并发脑梗死组 )ACEI/D基因多态性的ACE及AT1RA116 6C突变。结果 :EH组及EH并发脑梗死组的ACE基因的D等位基因频率为 5 0 %及 4 8% ,明显高于对照组的 33% (P <0 .0 5 )。AT1R基因的C等位基因频率在 3组之间差异无显著性意义 (P >0 .0 5 )。结论 :ACE基因可能是EH及EH并发脑梗死的重要遗传因素 。

Objective:To identify the I/D polymorphism of angiotensin converting enzyme (ACE) and A1166C polymorphism of angiotensin II type I receptor (AT 1R) genes in essential hypertension patients complicated by brain infarction or not. Methods:ACE and AT 1R genotyping with polymerase chain reaction (PCR) and PCR combined with restriction enzyme digestion were used to detect ACE gene I/ D polymorphism and AT 1R gene A1166C variations in 152 controls, 150 hypertensive subjects and 80 hypertensive patients with complicated by brain infarction. Results: The frequencies of the ACE D allele in groups of essential hypertension and complicated by brain infarction were higher than that in the health controls, respectively (P< 0.05). But there was no significant difference in the frequency of AT 1R C allele between the three groups (P> 0.05). Conclusion:ACE gene contributes to the development of essential hypertension and its complication of brain infarction, but the polymorphism of AT 1R gene is not associated with essential hypertension and its complication of brain infarction.