狗舌草提取物对L1210细胞的体外作用研究

Studies on Actions of Extract from Tephroseris Kirilowii Turcz. Holub to L1210 Cell Line in vitro

利用淋巴性白血病L1 2 1 0细胞 ,以单猪屎豆碱、槲皮素和环磷酰胺为参照 ,检查狗舌草60 %乙醇提取物 (EX)对L1 2 1 0细胞生长情况的影响 ;通过L1 2 1 0细胞台盼蓝拒染率的测定 ,研究EX对L1 2 1 0细胞活力的影响。结果发现 ,一定剂量的EX、单猪屎豆碱、槲皮素和环磷酰胺具有明显的体外抑制淋巴性白血病L1 2 1 0细胞的作用 ,且这种作用具有明显的剂量依赖性。其中EX和槲皮素在体外对L1 2 1 0细胞的细胞毒性较小 ,而单猪屎豆碱在体外对L1 2 1 0细胞的细胞毒性较大。

With a reference system of cyclophosphamide (CY), q uercetin (QU) and monocrotaline (MO) which are in the category of compounds in Tephroseris kirilowii Turcz. Holub, the actions of the extract from T. kirilowii with 60% ethanol solution (EX) to murine L1210 cell were studied in vitro in the aspects of cellular culture curve and Trypan Blue resisting rate. The results showed that the subjects at concentrations of 1 0 μg/ml(EX)?0.1μg/ml (MO) ?1.0μg/mL(QU) and 10.0μg/ml(CY)respectively gave certain effects against L1210 cells in repression of proliferation velocity and vigor (p<0. 01).