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大樱桃矮化砧吉塞拉(Gisela)的微体快繁技术体系研究 被引量:5

The Technical Research on Microsome of Sweet Cherry Dwarf Rootstock Gisela Fast Propagating System
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摘要 以从国外引进的大樱桃矮化砧吉塞拉 5、 6为材料 ,在MS +6 -BA1mg L +IBA0 1mg L培养基上诱导出丛生芽 ;在MS +6 -BA 0 5mg L +IBA 0 1mg L的增殖培养基上 3周增殖倍数可达 1 0倍 ;在 1 2MS+IBA 0 3mg L的生根培养基上生根率可达 70 %~ 1 0 0 % ;温室炼苗后 ,移栽成活率可达 90 %以上。 Using the imported Gisela Nos 5?6 ?7 as experimental seedling stock,studied complete sets of technology on micros ome of Gisela's dwarf rootstock propagating rapidly On the regenerat ive c ulture medium of MS+BA 1 mg/L+IBA 0 1 mg/L,its proliferows rate got up to 5~6 time as much after three weeks;Cultivated 25d of so on the rooting culture medium of 1/2 MS+IBA 0 3 m 9/2, its rooting rate reached ov er se venty percent; test-tube saplings having taken root were transplanted in soil te xture hased on river sand Small arch plastic shed in conservatory kept moisture and temperature, the survival rate of transplant came up to more than ninety pe rcent
出处 《中国农学通报》 CSCD 2003年第6期178-180,共3页 Chinese Agricultural Science Bulletin
基金 山东省科委科技攻关项目 ( 991 0 1 0 0 30 6 ) 山东省农业"三 0"工程项目
关键词 大樱桃 矮化砧 吉塞拉 GISELA 组织培养 技术体系 Sweet cherry Dwarf rootstock Gisela Tissue culture Propagation
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