男性不育患者生殖系感染、NO与细胞因子水平的关系

Study on genital infection ,level of NO and cytokine in infertile men

目的 在分子水平上进一步探讨男性生殖系感染解脲支原体和沙眼衣原体导致不育的机理 ;精浆一氧化氮的含量、IL - 1、IL - 2、IL - 6、IL - 10、IL - 12、TNF等细胞因子的水平在男性不育中的作用。方法 研究对象为选择 86例男性不育患者。采用PCR技术检测各组精浆中解脲支原体和沙眼衣原体DNA ;硝酸还原酶法测定精浆与血清中一氧化氮的含量 ;酶联免疫吸附法测定 5种白细胞介素和肿瘤坏死因子的水平。结果 男性不育伴有病原体感染组精浆与血清一氧化氮的含量为 135 4 2 μmol/L和 12 0 86 μmol/L ,明显高于男性不育无病原体感染组与对照组。且一氧化氮的含量与精子存活率、活动率呈负相关 ,而与畸形率呈正相关。不育患者精浆内细胞因子的变化为有病原体感染且一氧化氮的含量明显升高者IL - 1、IL - 6、TNF的含量高于无感染组和正常对照组 ,尤其是IL - 1和TNF(P <0 0 1) ,其次是IL - 6 (P <0 0 5 )。IL - 10和IL -12的含量下降 ,IL - 2R在各组间无明显差异。经相关分析发现精浆一氧化氮的含量与TNF呈正相关 (A =14 89,B =0 4 5 ,r=0 6 4 ) ,与IL - 10呈负相关 (A =17 0 8,B =- 0 0 6 ,r =- 0 5 6 )。结论 生殖系病原体感染影响体内一氧化氮、细胞因子的产生 ,使各细胞因子间网络平衡紊乱 。

Aim The role of genital infection,level of nitric oxide(NO) and cytokine in infertile men was studied.Methods The DNA of Ureaplasm urealyticum(Uu) and Chlamydia trachomatis(Ct) in seminal plasma were detected by polymerase chain reaction (PCR),the concentration of NO were done by nitric acid reductase;levels of IL-1,IL-2R,IL-6,IL-10,IL-12 and TNF in semen infectility patients were detected by ELISA.Results The NO levels in the semina and serum from the pathogens infection of male infertility was respectively 135.42μmol/L and 120.86μmol/L.Which was obviously higher than without infection of male infertility and normal fertility male.NO levels in seminal plasma correlated positively with abnormal morphology sperm and negatively with sperm motility.The levels of IL-1,IL-6,TNF in infertility male with genital infection was significantly higher and IL-10,IL-12 was lower than without infection and normal control groups.IL-2R level was similar in fertile and infertile men(P>0.05).Conclusion Effect of genital tract Uu,Ct infection on levels NO and cytokines.Leading to cytokines counterbalance disorder and abnormal reproduction.