散发性大肠癌癌组织18号染色体长臂微卫星改变的观察

Observation of microsatellite alterations on chromsome 18q in sporadic colorectal cancer

目的 探讨散发性大肠癌 (SCRC)癌组织 18号染色体长臂 (18q)微卫星改变 (MA)的发生情况及其在大肠癌发生中的意义。方法 采用聚合酶链反应 (PCR)、聚丙烯酰胺凝胶电泳、银染色方法 ,对 34例 SCRC患者的癌组织、癌旁组织及相应正常组织中 MA进行检测分析。结果 34例 SCRC患者中有 12例 (12 / 34)癌组织 18q上发生了至少一个位点的 MA,发生率为 35 .2 9% ,其中 D18S34、D18S4 73、D18S4 87、D18S5 8位点的 MA发生率分别为 2 0 .5 9% (7/ 34)、17.6 4 % (6 / 34)、11.76 % (4 / 34)、17.6 4 % (6 / 34)。仅 1例患者的癌旁组织在一个位点检测到 MA。结论 SCRC癌组织 18q上有较高频率的 MA,可能存在与大肠癌发生密切相关的基因 ;

Objective To detect the frequency of microsatellite alterations (MA)on 18q in sporadic colorectal cancer (SCRC) and the significance of MA in the progress of SCRC.Methods By polymerase chain reaction(PCR),polyacrylamide gel-electrophoresis and silver staining,MA was detected at 4 microsatellite loci on chromsome 18q in tumor tissues,adjacent tissues and normal colorectum tissue excised from 34 cases with SCRC.Results The cancer tissue from 12 cases demonstrated MA at one or more loci,the total frequency was 35.29% (12/34),the frequency of MA on D18S34,D18S473,D18S487,D18S58 was 20.59% (7/34),17.64% (6/34),11.76% (4/34) and 17.64% (6/34),respectively.In only one patient's adjacent tissue,MA was detected at one selected locus.Conclusions The most common MA occurrs at the 4 loci might imply the existence of the potential genes related to the carcinogenesis of colorectal cancer,and provide a effective index for diagnosis of colonrectal cancer.