摘要
用PCR-RFLP技术,对新疆分布的准噶尔雅罗鱼、贝加尔雅罗鱼和高体雅罗鱼的mtDNA D-loop高变区约827bp进行了扩增,用ScaI、HinfI、AluI、DdeI4种限制性内切核酸酶对56个样本的扩增产物酶切和RFLP分析,共检测到6种单倍型。准噶尔雅罗鱼存在2种单倍型:BDAA和BDBA;贝加尔雅罗鱼存在3种:AAAA、ABAA和ACAA;高体雅罗鱼只有1种。初步认为,准噶尔雅罗鱼、贝加尔雅罗鱼存在较丰富的群体内变异。用6种单倍型及净遗传距离绘制的UPGMA分子系统树,提示准噶尔雅罗鱼有可能是原始种,贝加尔雅罗鱼和高体雅罗鱼是由准噶尔雅罗鱼进化而来。
PCR-RFLP technique was employed to amplify about 827bp of mtDNA D-loop hypervariable region of Leu-ciscus baicalensis ,L. merzbacheri and L. idus in Xinjiang. The PCR products of 56 samples with four restriction enzymes were digested:Seal, HintI,AluI,Ddel,and RFLP analysis was done then. The study indicates that all of L. species and populations have six haplotypes, L. merzbacheri has two haplotypes: BDAA, BDBA; L. baicalensis has three: AAAA, ABAA, ACAA; L. idus has one:CAAA. It is primarily considered that L. merzbacheri and L. baicalensis have more intra-population mutations. The UPGMA trees with 6 haplotypes and net genetics distance pointed out that L. merzbacheri might be original species,L. baicalensis and L. idus are evolved from L. merzbacheri.
出处
《遗传》
CAS
CSCD
北大核心
2003年第4期414-418,共5页
Hereditas(Beijing)
基金
新疆石河子大学分子生物学实验室经费资助