NDRG1基因与肝组织分化及癌变的相关性

Correlation of NDRG1 gene with liver tissue differentiation and hepatocarcinogenesis

目的 :通过检测NDRG1基因在各种组织和细胞中的表达谱 ,探讨NDRG1与肝细胞分化及肝癌发生、发展的相关性。方法 :通过Northernblot和RT PCR方法检验NDRG1在肝癌和配对的非癌肝组织、正常肝、不同发育时期小鼠肝和人胎肝组织、人胚胎各组织以及多种细胞系中的表达谱。结果 :NDRG1在肝癌组织中的表达量显著高于配对的非癌肝组织和正常肝组织中的表达量 ;而正常肝组织与非癌肝组织相比 ,表达量差异无显著性。在所研究的 10种细胞系中 ,NDRG1的表达量在 2 93T人肾细胞系中最高 ,永生化人肝细胞系L0 2次之 ,在未分化的HLE肝癌细胞系及低分化的肝母细胞瘤HepG2中最低。NDRG1的表达量随婴鼠肝和人胎肝的发育而增高 ,至已达分化的成年肝又有所降低。结论 :NDRG1可能与肝细胞的分化相关 ,并在分化的一定阶段高表达 ,但不宜作为分化的标志物。NDRG1在肝癌中的高表达提示肝癌的形成不单是一种简单的去分化 ,其增殖。

Objective: To detect the expression profile of NDRG1 gene in different tissues and cell lines and explore the relationship of NDRG 1 with liver tissue differentiation and hepatocarcinogenesis. Methods: The expression profiles of NDRG 1 in hepatocellular carcinoma (HCC) tissues, paired noncancerous liver (PNL) tissues, adult normal liver (NL) tissues, baby mouse liver tissues and fetal liver tissues in different developmental stages and cultured cell lines were performed using RT PCR and Northern Blot analysis. Results: Expression of NDRG 1 was significantly up regulated in HCC tissues compared to that of NL tissues and PNL tissues, however, the expressions of NDRG1 in NL and PNL tissues showed no evident difference. In ten cell lines, the highest expression was in the 293T human kidney cell line, the next one in liver cell L02, and the lowest one in the undifferentiated HLE cell line. Expression of NDRG1 in liver tissues enhanced with the development of the baby mouse and human fetus. Conclusion: NDRG 1 is probably related to the liver cell differentiation and is highly expressed in the specific stage of the differentiation. However, it could not be recognized as a marker of differentiation. The high expression of NDRG 1 in HCC indicates that HCC is not just distributed to a simple de differentiation. Much more study is necessary in understanding the comprehensive relationship of the disorder proliferation and differentiation of HCC and the related genes.