参附注射液对幕上肿瘤手术患者血清SOD活性和MDA含量的影响

Effect of Shenfu injection on the superoxide dismutase activities and malondialdehyde levels in sera of supratentorial cerebral tumor patients during operation

目的 :研究参附注射液 (SF)对开颅手术患者脑内超氧化物歧化酶 (SOD)活性和丙二醛 (MDA)含量的影响 .方法 :4 0例ASAⅠ Ⅱ级幕上肿瘤行择期开颅手术患者 ,随机分为两组 ,Ⅱ组于切开硬脑膜后 ,用 9g·L-1生理盐水将 5 0mL参附注射液稀释至 2 0 0mL ,于 2 0min内输完 ,分别于输注前 (beforeinfusion :BI)、输注后即刻 (afterinfusion :AI)及输注 6 0min后 (6 0minafterinfusion :AI6 0 )三个时间点 ,采集颈内静脉球部血样 ,检测血清中超氧化物歧化酶 (SOD)的活性和丙二醛 (MDA)的含量 ,并记录对应时点的心率 (HR)、平均动脉压 (MAP) .Ⅰ组为对照组 ,在同一时间单纯输注 9g·L-1生理盐水 2 0 0mL ,在相同时间点抽取血样检测并记录HR及MAP .结果 :Ⅰ组输注前、输注后即刻及输注 6 0min后MDA含量无明显变化 (P >0 .0 5 ) .Ⅱ组输注前MDA含量明显低于输注 6 0min后 (P <0 .0 5 ) ,与输注后即刻比较无明显变化(P >0 .0 5 ) ;Ⅱ组输注前SOD活性明显低于输注 6 0min后 (P<0 .0 5 ) ,与输注后即刻比较无明显变化 (P >0 .0 5 ) ;Ⅰ组SOD活性输注前、输注后即刻及输注后 6 0min后均无明显变化 (P >0 .0 5 ) .组间比较 ,Ⅱ组MDA含量明显低于Ⅰ组 (P <0 .0 5 ) ,SOD活性明显高于Ⅰ组 (P <0 .0 5 ) .结论 :在颅脑手术?

AIM: To investigate the effect of ShenFu injection (SF) on the superoxide dismutase activities (SOD) and malondialdehyde (MDA) levels in patients undergoing craniotomy. METHODS: Forty ASA physical status Ⅰ～Ⅱ patients undergoing elective craniotomy for supratentorial cerebral tumor removal were randomized into 2 groups. Patients in group Ⅱ received SF 50 mL diluted with 150 mL of 9 g·L -1 NaCl within 20 min after opening the dura. Blood samples were taken from median cubital vein and internal jugular venous bulb for measuring SOD activities and MDA levels before infusion (BI), after infusion (AI) and 60 min after infusion (AI60), respectively. and heart rate (HR), Mean arterial pressure (MAP) were recorded at While patients in group Ⅰ received 200 mL of 9 g·L -1 NaCl at the same time. RESULTS: Compared with BI, MDA level was significant 200 mL of increased AI60 ( P <0.05), but no significant difference was observed between and AI in group Ⅰ( P >0.05). In group Ⅱ, there was no significant difference in MDA level between BI, AI, and AI60 ( P >0.05). Compared with BI, SOD activities was significantly increased AI60 ( P <0.05), but no significant difference was found between BI and AI in group Ⅱ ( P >0.05). No significant difference was found between BI, AI, and AI60 in group Ⅰ ( P >0.05). Sixty minutes after infusion, MDA level in group Ⅱ was significantly , lower and SOD activities was significantly higer ( P <0.05) , than that in group Ⅰ ( P < 0.05), respectively. CONCLU SION: Infusion of SF attenuates the formation of free radicals in patients undergoing craniotomy.