PCNA及AgNORs与涎腺腺样囊性癌远处转移和复发的关系

Relationship of the distant metastasis and recurrence of salivary adenoid cystic carcinoma to PCNA and AgNORs

目的 :探讨涎腺腺样囊性癌 (salivaryadenoidcysticcarcinoma ,SACC)细胞的增殖活性与远处转移和复发的关系 .方法 :应用抗增殖细胞核抗原 (proliferatingcellnuclearanti gen ,PCNA)单克隆抗体PC 10 ,采用免疫组织化学方法及Howell技术对 4 2例SACC石蜡标本进行PCNA和核仁组成区嗜银蛋白 (argyrophilicproteinsofthenuclearorganizerre gions,AgNORs)检测 ,并对临床资料进行了分析 . 结果 :SACC组织PCNA标记指数 (labelingindex ,LI)与相应的组织切片的AgNORs计数 (meannumbersofAgNORspernucleus)有显著相关性 (r =0 .5 2 8,P <0 .0 1) .PCNA表达和mAg NORs与SACC远端转移存在明显相关 (P <0 .0 1,P <0 .0 5 ) ,与病理分型存在相关性 ,实体型标本中PCNA表达和mAgNORs明显高于筛孔型和腺管型 (P <0 .0 5 ) .在随访 >10a病例中复发与无复发组PCNALI有显著性差异 (P <0 .0 5 ) ,与生存率无相关性 (P >0 .0 5 ) .结论

AIM: To investigate the relationship between the proliferative activity of salivary adenoid cystic carcinoma (SACC) cells and distant metastasis ,recurrence of the tumor. METHODS: Proliferating cell nuclear antigen (PCNA) and argyrophilic nucleolar organizer regions (AgNORs) in paraffin embedded SACC specimens from 42 cases were detected by immunohistochemical method using an anti PCNA monoclonal antibody and the technique of Howell.The clinical data of 42 cases were analyzed. RESULTS: PCNA labeling index (PCNALI) and the mean numbers of AgNORs per nucleus(mAgNORs) were well correlated ( r=0.528, P <0.01). The expression of PCNA and the mAgNORs had significant relation to distant metastasis of SACC ( P<0.01, P <0.05) and typing of pathology: The PCNALI and mAgNORs were higher in solid than in cribri/tubular from ( P <0.05). In the cases followed up>10 years, the PCNALI was correlated with rucurrence of SACC ( P <0.05). CONCLUSION: PCNALI and mAgNORs may be useful factors indicating distant metastasis of ACC.