SARS-CoV感染Vero E6细胞诱导细胞凋亡

SARS-CoV Infection Induces Apoptosis of Vero E6

为了确定SARS冠状病毒(SARS-CoV)感染Vero E6是否引起细胞凋亡,我们利用细胞DNA琼脂糖电泳,感染细胞的间接荧光染色和Hoechst 33258细胞核染色,以及流式细胞仪分析等方法证明了SARS-CoV感染的Vero E6具有典型的凋亡细胞学和生物化学特征。实验证明具有细胞凋亡特征的所有细胞均为处于感染晚期的细胞。表现明显细胞病变(CPE)的细胞大多已经出现核质凝缩或形成凋亡小体进入细胞凋亡的过程。可以断定SARS-CoV感染Vero E6细胞诱发了细胞凋亡。

To determine if SARS-associated coronavirus (SARS-CoV) induces apoptosis, we studied the SARS-CoV infected Vero E6 cells by agarose gel electrophoresis, indirect fluorescent staining for infection and Hoechst 33528 staining for nuclei, as well as by flow cytometry. It was found that SARS-CoV infected Vero E6 cells showed typical apoptosis characteristics. All the typical apoptotic cells were in late-infection period. Most of the cells with cytopathic effect (CPE) showed nuclei with condensed chromatin or fragmented into apoptotic bodies. It was suggested that SARS-CoV infection induces apoptosis of Vero E6.