甜菜夜蛾核多角体病毒sod基因的克隆及原核表达

Cloning and Expression of Superoxide Dismutase Gene Encoded by Spotoptera exigua Multi Nucleopolyhedrovirus

甜菜夜蛾核型多角体病毒中国株(Spotoptera exigua MNPV-Z)超氧化物歧化酶基因(sod)业已被克隆及在大肠杆菌中进行了表达,证明了SeMNPV-Z的sod基因产物确有SOD活性,其活力单位约为291.19U/mL培养液。DNA测序结果表明SeMNPV-Z的sod基因编码151个氨基酸,与人的sod1基因的核苷酸的同源性为50％,与LdNPV、HaSNPV、HcNPV、AcNPV和BmNPV的sod基因的同源性分别为64％、63％、63％、65％、63％。

Superoxide dismutases scavenge superoxide radicals and protect cells from oxidative stress.A sod gene of Spotoptera exigua Multi Nucleopoleohedrovirus (SeMNPV) from China has been cloned and expressed in E.coli with the activity of SOD being 291.19U/mL. DNA sequence analysis showed that sod gene of SeMNPV encoded 151 amino acids. The sequences homology between SeMNPV sod gene and human sodl gene was 50%, and 64% between SeMNPV and LdNPV, 63% between SeMNPV and HaSNPV, HcNPV, BmNPV, 65% between SeMNPV and AcNPV.