儿童肾小球疾病不同标本转化型生长因子-β_1检测结果的比较

Comparative analysis of determination of TGF-β_1 level in various body fluid in children with glomerular diseases

目的 比较评价儿童肾小球疾病血浆、血清、尿液不同标本的转化型生长因子 β1(TGF β1)水平。方法 用ELISA方法测定 30例轻微病变、30例系膜增生性肾小球肾炎、2 4例IgA肾病、2 4例局灶节段性肾小球硬化患儿和 30例健康对照组儿童血浆、血清、尿液中的TGF β1水平。结果 尿液标本的TGF β1水平对各组的诊断有显著意义 (P <0 0 5 ) ,而血浆、血清标本没有诊断意义 (P >0 0 5 )。血浆测定TGF β1灵敏度较血清好。血浆、尿液指标有较好的相关性 (r=0 85 ,P <0 0 5 ) ;血清与尿液仅有一定的相关性 (r=0 5 3,P <0 0 5 ) ;血浆与血清无显著相关性 (r =0 4 9,P >0 0 5 )。结论 尿液为检测肾炎患儿TGF β1水平的较好指标 ,血浆次之 ,血清最差。

Objective To compare the TGF-β 1 level in serum,plasma and urine in children with glomerulonephritis and to decide which sample offer the best index to determine TGF-β 1 level.Methods The plasma,serum and urinary TGF-β 1 levels were measured in 30 patients with mild lesion (ML),30 with mesangial proliferative glomerulonephritis(MsPGN),24 with IgA nephropathy(IgAN),24 with focal segmental glomerular sclerosis(FSGS) and in 30 healthy controls by sensitive enzyme-linked immunosorbent assay (ELISA).Results Urinary index was significant in diagnosing cases of the five groups(P<0.05),while,plasma and serum index were not significant(P>0.05).The sensitivity of the TGF-β 1 level in plasma samples was better than that in serum.The TGF-β 1 level in plasma and urinary samples had significant correlation(r=0.85,P<0.05);seurm and urinary indexes had some correlation(r=0.53,P<0.05);however,plasma and serum had no correlation(r=0.49,P>0.05).Conclusion Urinary sample is a preferable index to determine TGF-β 1 level for glomerular diseases,and plasma and urinary samples are second to it respectively.