摘要
利用农杆菌介导的遗传转化系统 ,将白叶枯病抗性基因Xa2 1导入山东主栽品种圣稻 30 1胚性愈伤组织 ,从T0 至T5代 ,利用GUS染色和分子标记辅助逐代选择并进行田间抗病虫性鉴定 ,获得了大量纯合稳定的转基因圣稻 30 1。
Using a genetic transformation system mediated by Agrobacterium tumefaciens, Xa21 gene against the bacterial blight disease was introduced into calli of a japonica variety Shengdao301 cultivated in Shandong Province. Based on Gus detection, marker assisted selection and inoculation from T_0 to T_5 generation, a great number of homozygous transgenic Shengdao301 plants were obtained. Performance of field resistance and agronomy traits were investigated.
出处
《山东农业科学》
2003年第6期10-11,14,共3页
Shandong Agricultural Sciences
