河南食管癌高发区老年与青年食管癌患者P53蛋白和Rb蛋白表达变化

Changes of P53 and Rb proteins expression in esophageal squamous cell carcinoma of the elderly and the young patients at high-risk area for the cancer in Henan Province

目的 探讨食管癌高发区林州市 (原林县 )老年食管癌患者的分子学变化特征及其意义。 方法 采用免疫组化卵白素 生物素 过氧化物酶复合物 (ABC)法和组织病理学方法 ,分析食管鳞癌组织中P5 3和Rb蛋白的表达状况与病变程度和年龄差异之间的关系。 结果 老年食管癌患者食管癌组织P5 3变化阳性率 (2 7/ 33,82 % )明显高于同一地区青年患者 (10 / 4 3,2 3% ) (P <0 0 0 1)。老年患者Rb变化阳性率亦高于老年组 ,但差异无统计学意义。此外 ,老年患者P5 3和Rb在食管癌组织中的表达具有较高的一致性 (P <0 0 1)。 结论 本研究结果为进一步探讨食管癌高发区河南林州市老年患者食管癌分子发病机制提供了资料。

Objective To characterize the molecular changes and their significance in pathogenesis of esophageal squamous cell carcinomas from the elderly patients (≥60 years old) at high risk area of esophageal cancer in Linzhou, Henan Province. Methods Immunohistochemical method(ABC) and histopathological method were applied to examine the expressions of P53 and Rb proteins in esophageal squamous cell carcinomas and the relationship of the expressions with the lesions and age distribution was analyzed. Results Positive immunostaining rate for P53 was much higher in esophageal cancer tissue of the elderly patients(27/33, 82%) than that of the young patients (10/43, 23%) ( P <0 001). Although the positive immunostaining rate for Rb of the tissue from the elderly patients was higher than that of the young patients, the difference was not significant. In addition, the high coincident alteration for the positive immunostaining rate for P53 and Rb was observed in the elderly patients with the cancer. Conclusions The results provide some information for further study of molecular mechanisms involved in the elderly patients in Henan, the high incidence area for esophageal cancer.