永椿香槐组织培养研究

A Study on Tissue Culture of Cladrastis yunchunii

经过愈伤组织的诱导 ,不定芽和根系的培养 ,最终培养形成永椿香槐完整植株 .经过筛选 ,使用新长出的茎段作为外植体培养材料 ,在MS +6 -BA 1 5 0mg/L +NAA 0 2 0mg/L +3 %蔗糖培养基中培养愈伤组织和不定芽 ,在White +IBA 1 0 0mg/L +1 5 %蔗糖培养基中诱导根突 ,在 1 / 2MS +IBA 1 0 0mg/L +1 5 %蔗糖培养基中培养根 ,是永椿香槐组织培养最适宜的组合 .

Full plants of Cladrastis yunchunii are obtained by tissue culture through inducement of callus as well as culture of adventitious buds and roots.It is the best combination during this tissue culture of Cladrastis yunchunii that selecting new stem segments as explants,MS+6-BA 1.50 mg/L+NAA 0.20 mg/L+3% sucrose as culture medium for culturing callus and adventitious buds, White+IBA 1.00 mg/L+1.5% sucrose as culture medium for inducing root tips,1/2 MS+IBA 1.00 mg/L+ 1.5% sucrose as culture medium for culturing roots.