人参果组织培养技术研究

A Study on Culture Technology of Tissue of Solanum muricatum

以人参果的幼嫩茎段和叶片作外植体 ,在MS基本培养基附加不同浓度 6 -BA +IAA ,6-BA +NAA和KT +2 ,4D组成的固体培养基中进行培养 .结果显示 :MS +6 -BA 0 0 7mg/L +IAA 0 0 3mg/L ,MS +6 -BA 0 70mg/L +IAA 0 0 3mg/L ,MS +6 -BA 0 70mg/L +IAA 0 0 5mg/L ,MS +6 -BA 1 0 0mg/L +IAA 0 0 5mg/L 4种培养基中的材料愈伤组织长势比较好 ,分化明显 .经继代培养 ,丛生芽大量形成 ,丛生苗根系发达 .其他培养基则停留在愈伤组织阶段或只长出极少数短的根 .

The delicate stem cuttings of Solanum muricatum. were inoculated on MS base medium supplied with different concentrations of 6-BA and IAA. The results showed that, the differentiation of callus on the MS+6-BA 0.07mg/L+IAA 0.03 mg/L, MS+6-BA 0.70 mg/L + IAA 0.03 mg/L,MS+6-BA 0.70 mg/L+IAA 0.05 mg/L,MS+6-BA 1.00 mg/L+IAA 0.05 mg/L were obvious. By continuing culture, the number of roots increased and grew well.The other stem cuttings were difficult to induced to callus or few roots were induced