我国五省美洲钩虫COI基因序列多态性分析

Polymorphism Analysis of Cytochrome C Oxidase 1 (COI) Gene in Necator americanus Collected from 5 Provinces in China

目的 测定和比较四川、海南、云南、湖北、江苏五省美洲钩虫细胞色素氧化酶Ⅰ(COI)基因序列,分析基因序列的多态性。方法 应用PCR技术扩增美洲钩虫COI基因,并进行DNA测序和分子进化分析。结果 五省的美洲钩虫COI基因序列相似性为97％～99％,但在其PCR扩增获得的595bP的COI基因片段上,共检出19个核苷酸位点上的变异,变异位点上转换明显多于颠换,基因序列差异值为1.34％～2.18％。结论 五省的美洲钩虫COI基因序列具有很高的相似性,但特定碱基位点仍存在一定差异。

Objective To detect and compare the COI gene sequences of Necator americanus collected from the provinces of Sichuan, Hainan, Yunnan, Hubei and Jiangsu, and to analyze the genetic diversity of the geographic isolates. Methods COI genes of N. americanus were amplified from the genomic DNA by PCR and sequenced. Results The COI gene sequences of N. americanus from five provinces were 97%-99% identical over 595 bp, and base variation oc- curred in 19 nucleotide sites in which the transition was more frequent than the transversion. The difference between the se- quences ranged from 1. 34% to 2. 18%. Conclusion The COI gene sequences show high identity among the geographic isolates of N. americanus with some difference at specific nucleotide sites.