SSA抗原及其不同阳性表位的临床意义

The clinical significance of SSA antigen and its different positive expressions

目的 区别与SSA抗原不同阳性表位结合的抗SSA抗体在疾病中对机体损伤的不同性 ,探讨抗 60 0 0 0SSA抗体在相关自身免疫病中的致病作用。方法 用按已知 60 0 0 0SSA抗原多肽链中 2 0个阳性表位和 1个阴性片段的氨基酸序列人工合成的不同表位寡肽 (MAPs)为抗原 ,以ELISA方法检测 59例抗SSA抗体阳性的患者血清 ,分析引起机体不同损伤的抗SSAMAPs抗原抗体的特点。结果 抗 60 0 0 0SSA抗体是一种多克隆抗体 ,不同病人所具备的抗体与 60 0 0 0SSA抗原发生免疫反应的表位可以不同。抗 2 1个MAPs抗体的阳性与否和皮肤损伤无明显相关性 ;抗 2、1 4、1 5、2 1号MAPs抗体阳性与否与唾液腺损伤具有正相关性 ;抗 7、1 6号MAPs抗体阳性与否和肾脏损伤具有负相关性 ;抗 6号MAPs抗体阳性与否和心脏损伤具有负相关性。结论 某些MAPs抗体的出现提示一定器官受损 ;某些MAPs抗体的出现则对一定的器官具有保护作用。由此提示 :可能是不同病人具有抗不同MAPs的抗体 。

Objective To distinguish the difference among the pathogenesis of 60 000 SSA antigen mono antigen peptides (MAPs), and to discuss the nosogenesis of anti 60 000 SSA antibodies in correlative rheumatic diseases. Methods MAPs were artificially synthesized according to the amino acid sequence of 20 positive epitopes and 1 control segment of 60 000 SSA antigen. ELISA against recombinant 60 000 SSA antigen MAPs were done to detect anti MAPs antibodies in 59 sera with anti SSA antibodies, and analyzing the relations of anti MAPs antibodies and organism injuries. Results Anti 60 000 SSA antibodies are multiple clone autoantibodies. Different patients have different immunal response to MAPs. Anti MAP 1～21 antibodies have no relation to skin lesion; anti MAP 2,14,15,21 antibodies have positive relation to salivary gland lesion; anti MAP 7,16 antibodies have negative relation to kidney lesion; anti MAP 6 antibodies have negative relation to heart lesion. Conclusions The appearance of some anti MAPs antibodies implies the lesions of some organs, while the appearance of some other anti MAPs antibodies have the protection to some organs. We concluded that it is maybe the different anti MAPs antibodies that result in different clinical manifestations.