人眼组织中共刺激分子、Fas/FasL及主要组织相溶性复合体类分子的免疫组织化学研究

Immunohistochemical studies on the expression of costimulatory molecules, Fas/FasL, and majorhistocompatibility complex Ⅱ antigens in normal ocular tissues

目的 探讨正常人眼组织 (虹膜睫状体、脉络膜、视网膜 )中共刺激分子 B7- 1和 B7- 2、与凋亡相关的分子 Fas/ Fas L、人类白细胞抗原 ( human leukocyte antigen,HL A) - DR及 CD6 8(抗巨噬细胞抗体 )分子的表达及意义。 方法 12只正常供体人眼 (死后 16～ 2 4h)均来自中山眼科中心眼库 ,将 6只供体眼组织 (虹膜睫状体、脉络膜、视网膜 )行常规冰冻切片 ,另 6只做视网膜平片 ,分别于各部分眼组织切片及视网膜平片进行免疫组织化学研究 ,探讨正常人眼组织中 B7- 1、B7- 2、HL A- DR、CD6 8、Fas/ Fas L分子的表达。 结果 人虹膜睫状体中有 B7- 2、Fas L、CD6 8、HL A- DR分子的表达 ;脉络膜中有 B7- 1、Fas L、CD6 8、HL A- DR分子的表达 ;视网膜中可见 HL A- DR、CD6 8、Fas L分子的表达 ,而无 B7- 1、B7- 2、Fas分子的表达。 结论 共刺激分子 B7- 1和 B7- 2、Fas/ Fas L及主要组织相溶性复合体 ( major histocompatibility com-plex,MHC- )类分子在不同人眼组织中的表达不同 ,这些分子的表达可能对维持人眼组织免疫环境的稳定性起着重要作用。

ObjectiveTo investigate the expression of costimulatory molecules, Fas/FasL, and major histocompatibility complex (MHC)-class Ⅱ antigens in normal ocular tissues.MethodsTwelve eyes were obtained from the eye bank of Zhongshan Ophthalmic Center within 16 to 24 hours postmortem. Six eyes were used for making the retinal wholemounts, and the tissues (iris and ciliary body, choroid, and retina) of the others were used for making the frozen sections. Immunohistochemical staining was carried out on these retinal wholemounts as well as on tissue sections to investigate the exprenion of B7-1 and B7-2 (costimulatory molecules), HLA-DR(MHC class Ⅱ), CD68 (macrophages), Fas/FasL.ResultsThe results of immunohistochemical staining showed the presence of B7-2, FasL, CD68 and HLA-DR in the iris and ciliary body. Expression of B7-1, FasL, CD68, and HLA-DR was found in the choroid while HLA-DR, CD68 and FasL were detectable in the retina.ConclusionExpression of costimulatory molecules, MHC-class Ⅱ molecules and molecules related to apoptosis is different in the iris, ciliary body, choroid, and retina, which may play an important role in the stability of the immunological microenvironment of these tissues.