摘要
利用半定量RT-PCR方法 ,根据中国对虾(Fenneropenaeuschinensis)抗菌肽的cDNA序列 ,设计一对特异引物 ,首次对中国对虾养殖期 (PL11期仔虾～稚虾 )抗菌肽的表达进行了研究。结果在各时期样本所提取的总RNA中 ,均扩增出一条长度约为200bp的特异性条带 ,表明中国对虾养殖期不同阶段均有抗菌肽的表达 ,提示中国对虾抗菌肽基因为组成型表达 ,并可能是中国对虾抵抗微生物感染的一个重要分子基础。
Antimicrobial peptides are important for non-specific host defence in many animals. This research considers the developmental expression of antimicrobial peptide gene in maturation period of Fenneropenaeus chinensis. Total RNA samples from different developmental stages (PL11-juvenile shrimp) were isolated and relative mRNA contents were detected using the semi-quantitative RT-PCR method. Results showed that antimicrobial peptide transcripts were present in all detected samples during different developmental stages from PL11 to adult shrimp. This suggests that antimicrobial peptide is constitutively expressed in shrimp Fenneropenaeus chinensis and it may also play an important role in innate defense against infection.
出处
《海洋科学》
CAS
CSCD
北大核心
2004年第1期48-51,F003,共5页
Marine Sciences
基金
国家重点基础研究专项经费(G1999012007)
国家自然科学基金资助项目(30140017)
欧盟第五框架协议项目(ICA4-CT-2001-10023)
关键词
中国对虾
抗菌肽
半定量RT
基因
组成型
shrimp (Fenneropenaeus chinensis)
antimicrobial peptide
semi-quantitative RT-PCR
