马立克氏病病毒pp38基因启动子和增强子的克隆和序列分析

Cloning and Sequencing Analysis for Promoter-enhancer Region of pp38 Genes from Different Strains of Marek's Disease Virus

参考GeneBank发表的马立克氏病病毒(MDV)国际标准强毒株GA的基因序列,设计合成一对引物,分别以RB1B,814,GD2(广东分离株),J 1 E(北京分离株),Md11,Md5,CVI988等不同毒株的MDV基因组DNA为模板,通过PCR扩增,获得了预期大小的PCR产物。该产物经pGEM T easy克隆后测序,将所得序列进行比较分析。结果发现:不同毒株间pp38基因的启动子和增强子序列间有缺失突变,序列的同源性大于95 9%,其中大多数的突变发生在MDV复制的原点附近。

The promoter-enhancer region of the pp38 phosphoprotein were amplified from genomic DNA of MDV RB1B,GD2,J-1-E,Mdll,Md5 and CVI988 strains by PCR technique.The amplified fragments were cloned into pGEM-T-easy vector,and the obtained positive clones were sequenced and compared with each other.This study shows that there are some absent mutants.The homology of these genes was more than 95.9%,and that most of the mutants are near the replication origin of MDV itself.