NOV对人神经干细胞增殖和分化的影响

Effects of NOV protein on proliferation and differentiation of human neural stem cells

目的 探讨NOV蛋白对人神经干细胞 (HNSCs)增殖和分化的影响。 方法 NOV基因重组质粒转染COS 7细胞 ,收集COS 7细胞和NOV基因修饰COS 7细胞的条件培养液 (COS CM和NOV CM ) ,作用于培养的HNSCs,3H TdR掺入液闪检测HNSCs的增殖 ,免疫细胞化学和流式细胞仪检测HNSCs的分化。 结果 (1)NOV CM能明显促进HNSCs对3H TdR的摄入 ,说明NOV CM能明显促进HNSCs的增殖 ,另外NOV CM的促细胞增殖作用具有一定的量效关系 ;(2 )免疫细胞化学和流式细胞仪结果显示 ,NOV CM促进HNSCs向神经元方向分化。结论 NOV蛋白可能具有促进HNSCs增殖和分化的作用。

Objective To explore effects of nephroblastoma overexpression gene(NOV) protein on proliferation and differentiation of human neural stem cells(HNSCs). Methods After NOV was transfected into COS-7 cells with liposome method, conditioned mediums(CMs) of COS-7 cells and COS-7 cells modified by NOV gene were collected (COS-CM and NOV-CM, respectively). Proliferation of HNSCs cultured in the CMs was detected with incorporation of 3 H-TdR, and differentiation of HNSCs was examined by using immunocytochemistry and flow cytometry. Results (1)NOV-CM markedly promoted incorporation of 3 H-TdR to cultured HNSCs in a concentration-dependent manner, indicating that NOV-CM promotes proliferation of HNSCs. (2) NOV-CM increased differentiation of HNSCs to neurons, but decreased it to astrocytes. Conclusion NOV protein could promote proliferation and differentiation of HNSCs.