三参滋胃饮对慢性萎缩性胃炎大鼠胃黏膜G细胞和D细胞的作用

Effects of Sanshen Ziwei Yin on G cells and D cells of gastric mucosa in rats with chronic atrophic gastritis

目的 :探讨三参滋胃饮对慢性萎缩性胃炎 (CAG)大鼠胃黏膜 G细胞和 D细胞的作用及其机制。方法 :采用综合法 (主动免疫、寒凉胆汁及热水灌胃等 )复制 CAG大鼠模型 ,应用大、小剂量三参滋胃饮及对照药物维酶素 (WMS)连续给药 5 0 d,观察给药前后各组胃黏膜病理改变、免疫组化染色后 G细胞及 D细胞的形态和数量变化。结果 :萎缩性胃炎模型鼠 G细胞和 D细胞数量明显减少 (P<0 .0 1 ) ,且两种细胞均有脱颗粒现象 ;G/D比值下降 ,表明在萎缩发生期间 G细胞下降速度较 D细胞快 ,即 G细胞对萎缩病变反映更为敏感。给药结束后三参滋胃饮组 G、D细胞数量均明显增多 ,最终使 G/D比值恢复至正常组水平 ,且与 WMS组比较差异有显著性(P<0 .0 5 )。大剂量组病理改变的恢复情况优于小剂量组。结论 :三参滋胃饮可能通过改善胃黏膜炎症反应促进腺体及内 (旁 )分泌细胞再生 ,通过改善胃肠激素对胃功能的调节作用而发挥治疗作用。

Objective To study the effects and mechanisms of Sanshen Ziwei Yin(SZY) on D cells and G cells of gastric mucosa in rats with chronic atrophic gastritis (CAG). Methods Rat models with CAG were established with synthesis method (active immunity,cold and cool gall and hot water irrigation). High and low dose of SZY and Weimeisu (WMS,as control) were used for 50 days constantly. The pathological changes of gastric mucosa,morphological and number changes were observed before and after treatment. Results The number of G cells and D cells of rats with CAG decreased significantly ( P <0 01), and degranulation presented in both two kinds of cells. The decrease of G/D ratio indicated that G cells descented more fast than D cells, that is to say, G cells were more sensitive to CAG. After treatment,the number of D cells increased obviously in treatment group, and apporached to normal levels at last,there was significant difference compared with WMS group. Conclusion SZY may have therapeutic effects on CAG through improving the inflammatory reaction of gastric mucosa to promote the regeneration of gland, endocrine and paracrine cells,and improving the regulation effects of gastrointestinal hormones on gastric function.