分枝扩增法检测鼻咽癌患者外周血EB病毒基因

Detection of EB virus in peripheral blood of patients with nasopharyngeal carcinoma by ramification amplification method

目的 :应用分枝扩增法 (RAM)检测鼻咽癌患者外周血 EB病毒基因。方法 :利用人工合成的 EB病毒 EBER- 1靶基因序列 ,建立分枝扩增的方法。以 EB病毒阳性细胞株 Raji和 EB病毒阴性的人白血病细胞株 NB4作对照 ,检测鼻咽癌患者外周血中 EB病毒基因 ,并用 PCR方法作对比。结果 :RAM法能够检测样品中 1 0个分子的靶基因 ,32例鼻咽癌患者标本中 ,2 8例阳性 (87.5 % ) ,与 PCR法的检测结果相一致。结论 :RAM法敏感、特异。

Objective To detect EB virus in peripheral blood of patients with nasopharyngeal carcinoma by ramification amplification method (RAM). Methods RAM was used to detect EB virus with the synthetic target gene sequence of EBER 1. EB viruses in some cases of patients with nasopharyngeal carcinoma were detected by RAM and PCR, respectively, and the result of RAM was compared with that of PCR. Raji cells and NB4 cells were used as positive control and negative control respectively. Results RAM could detect 10 molecular target gene sequence of the samples, 28 cases were positive in 32 patients with nasopharyngeal carcinoma and the positive rate was 87 5%, The result was identical with that of PCR. Conclusion RAM is sensitive and convenient, and also easy to perform.