黄连体细胞胚胎发生的同步控制

SYNCHRONIZATION OF SOMATIC EMBRYOGENESIS IN COPTIS CHINENSIS F.

本文采用物理、化学方法以及生长调节剂与过筛相结合的综合法,对黄连体细胞胚胎发生进行了同步控制。结果发现:综合法效果最理想,经2,4-D诱导形成体胚后,转到ABA+NAA的MS培养基上培养,然后分级过筛,再继代培养,可使体胚同步率明显增高,达到60—85%,体胚发育也正常。由此我们认为:体细胞胚胎发生同步控制的关键问题,是用植物生长调节剂的种类与浓度的配合使用来调节体胚发育,分级过筛只是一种获得体积大小一致的体胚的方法。

This paper represented the synchronization of somatic embryogenesis of Coptis chinensis F. by using physical and chemical methods as well as a comprehensive method——plant growth regulators supplemented with fractional sieve. The results have shown that the comprchensive method was thebest one compared with the physical or chemical methods. The somatic embryos of C. Chinensis were induced on basal MS medium containing 0.5mg/L2, 4-D+0,2mg/L NAA, then transferred into the MS medium supplemented with 0.5mg/L ABA+0.2mg/L NAA subcultured for two weeks. After the somatic embryos fractionated with different mesh size of sieves, 60—85% of synchronous rates of somatic embryos were achieved in this method. From this study, we can get a conclusion: the key point in plant somatic embryogenesis and synchronization is to control the development of somatic embryos by using correct kinds of and suitable concentrations of plant growth regulators. To use fractional sieve is only a method for getting identical size of somatic embryos.