农垦58s育性转换与吲哚乙酸氧化酶和过氧化物酶的关系

STUDIES ON RELATIONS BETWEEN FERTILITY TRANSFORMATION OF NONGKEN 58s AND IAA OXIDASE AND PEROXIDASE

本文报道了农垦58s和农垦58幼穗发育期长短日下吲哚乙酸氧化酶和过氧化物酶的活性变化。结果表明:58s LD叶片中吲哚乙酸氧化酶活性从花粉母细胞形成期至花粉内容物充实期显著低于58s SD,与游离IAA的积累密切相关,可能影响花粉育性;但过氧化物酶的活性在上述时期显著高于58s SD,与游离IAA的积累无关,其作用尚不清楚。58s LD幼穗及花药中两酶活性在减数分裂期和花粉内容物充实期均较58s SD高,与同期幼穗及花药中游离IAA的亏缺有一定联系,从而可能在一定程度上影响花粉育性。对照农垦58叶片和幼穗及花药中两酶活性变化都不呈现上述现象。

It is reported in the paper the study on the changes of IAA oxidase and peroxidase activities in Nongken 58s and control Nongken 58, during their panicle development under 14-hours sunshine a day (long day, LD)and 10-hours sunshine a day (short day SD). The results are as follows: The IAA oxidase activity in leaf of 58s LD is much lower than that of 58s SD from the formation stage of pollen mother cell to the filling stage of pollen, this makes the free IAA accumulated in leaf of 58s LD and maybe affect the pollen fertility; however, the peroxidase activity in leaf of 58s LD is much higer than that of 58s SD during the same panicle development periods, and this has nothing to do with the accumulation of free IAA in leaf of 58s LD, its effect is not known. The activities of two enzymes in panicle and anther of 58s LD are higer than those of 58s SD at the stage of meiotic division of pollen mother cell and the filling stage of pollen, this has some thing to do with the destruction of free IAA in panicle and anther of 58s LD, they may affect the fertility of 58s to a certain extent. The phenomena above l(?)ve not been observed for two enzymes in leaf, panicle and anther of control Nongken 58.