试管苗嫩枝顶端嫁接技术的研究

STUDY ON TIP GRAFTING IN VIVO BY USING IN VITRO PLANTLETS

根据植物顶端生长优势的原理,在猕猴桃(Actinidia chinensis)胚乳培养中,将愈伤组织上分化的芽,切割下来作接穗,利用简单装置,进行“嫩枝顶端嫁接”,嫁接成活率高达90%左右,并至少提前1—2年开花、结果。利用嫩枝顶端嫁接技术,不仅提高了试管苗的成活率,而且在简化培养程序、缩短培养周期、降低培养成本上都有明显的效果。

Based on the mechanism of plant tip growth potential plantlets regenerated from Actinidia chinensis endosperm culture in vitro as scions were grafted on 1—2 years seedings or juvenile vine of fruit plants. Survival rate can reached to 90% and the grafting plants flowered 1—2years in advance compared with the control plants.Tip grafting in vivo by using in vitro plantlets not only increased survival rate, but also simplified cultural expenses.The tip grafting is significant in fruit trees either conventional breeding or biotechnological breeding.