基质金属蛋白酶表达在皮肤光老化皱纹形成中的作用

Relationship Between Expression of Matrix Metalloproteinases and Wrinkle Formation in Photoaging Skin

目的探讨皮肤光老化皱纹形成与真皮成纤维细胞基质金属蛋白酶(MMP)-1,MMP-3mRNA及其组织抑制剂(TIMP)-1表达的关系.方法采用原位杂交和免疫组化的方法检测紫外线光化学疗法(PUVA)治疗期间及治疗后不同时间银屑病患者背部非皮损区真皮成纤维细胞MMP-1,MMP-3mRNA及TIMP-1蛋白的表达并定量分析.结果在PUVA治疗期间、治疗后1～6个月及治疗后6个月以上的银屑病患者背部非皮损区真皮成纤维细胞均持续表达MMP-1、MMP-3 mRNA,而TIMP-1蛋白仅在治疗期间一过性轻度表达.结论PUVA治疗引起的皮肤光老化皱纹形成可能与真皮成纤维细胞基质金属蛋白酶及其组织抑制剂表达失衡密切相关.

Objective To investigate the relationship between expression of matrix metalloproteinases of dermal fibroblasts and wrinkle formation in photoaging skin.Methods In situ hybridization histochemistry and immunocytochemistry were used to detect the expression of matrix metalloproteinases MMP-1,MMP-3mRNA and the expression of tissue inhibitor of MMP-1at(TIMP-1)protein in dermal fibroblasts from non-lesional skin of psoriatic patients during and after PUVA treatment.Results The expression of MMP-1and MMP-3mRNA of dermal fibroblasts from non-lesional skin was persistently up-regulated during PUVA thera-py,and lasted more than6months after treatment;while the expression of TIMP-1protein was only slightly expressed for a short period during PUVA therapy.Conclusion Wrinkle formation in photoaging skin after PUVA therapy is correlated with the imbalance of expression of matrix metalloproteinases and their inhibitors of dermal fibroblasts.