Cloning and Expression Analysis of Violaxanthin De-Epoxidase (VDE) cDNA in Wheat 被引量：1

小麦紫黄质脱环氧化酶(VDE)cDNA的克隆及表达(英文)

下载PDF

导出

摘要 Violaxanthin de-epoxidase (VDE) is the key enzyme in the xanthophyll cycle and protects plant photosynthetic apparatus from the damage of excessive light. A wheat (Triticum aestivum L cv. Xiaoyan 54) VDE cDNA was obtained using RT-PCR method. Its deduced protein sequence shares high identity with that of Arabidopsis and rice. Southern blot revealed that there are three copies of VDE gene per haploid genome of wheat. VDE transcript levels were higher in green leaf than in root, seed and etiolated leaf. Northern blotting analysis indicated that VDE mRNA level is induced during greening process of etiolated wheat seedling and increased by intense light illumination. 应用RT-PCR方法从小麦(Triticum aestivum L.cv.Xiaoyan 54)中克隆了紫黄质脱环氧化酶(violaxanthinde-epoxidase,VDE)cDNA,预测的蛋白质序列与拟南芥(Arabidopsis thaliana)、水稻(Oryza sativa)有很高的同源性。Southern杂交结果表明,在小麦中可能存在3个拷贝的紫黄质脱环氧化酶基因。Northern杂交结果表明它在绿色叶片中特异表达,在黄化小麦幼苗变绿过程中其mRNA水平受光诱导,并且强光增加了其mRNA在成熟叶片中的表达。

作者张吉军英加常胜合李滨李振声

机构地区中国科学院遗传与发育生物学研究所植物细胞与染色体工程国家重点实验室

出处《Acta Botanica Sinica》 CSCD 2003年第8期981-985,共5页 Acta Botanica Sinica（植物学报：英文版）

基金国家重点基础研究发展规划项目(Gl1995010200)

关键词 violaxanthin de-epoxidase xanthophylls cycle PHOTOPROTECTION Triticum aestivum 紫黄质脱环氧化酶叶黄素循环光保护小麦克隆表达

分类号 S512.1 [农业科学—作物学] Q943.2 [生物学—植物学]

引文网络
相关文献

参考文献9

1林荣呈,李良壁,匡廷云.水稻紫黄质脱环氧化酶基因的克隆、体外表达及酶促反应[J].科学通报,2002,47(6):449-451. 被引量：5
2李滨,邹琦,李全梓,崔家骏.小麦紫黄质脱环氧化酶基因WVDE序列(英文)[J].植物生理与分子生物学学报,2001,27(4):359-360. 被引量：1
3David A. Hieber,Robert C. Bugos,Amy S. Verhoeven,Harry Y. Yamamoto. Overexpression of violaxanthin de-epoxidase: properties of C-terminal deletions on activity and pH-dependent lipid binding[J] 2002,Planta(3):476～483
4Amy S. Verhoeven,Robert C. Bugos,Harry Y. Yamamoto. Transgenic tobacco with suppressed zeaxanthin formation is susceptible to stress-induced photoinhibition[J] 2001,Photosynthesis Research(1-2):27～39
5Sue-Hwei Chang,Robert C. Bugos,Wen-Hao Sun,Harry Y. Yamamoto. Antisense suppression of violaxanthin de-epoxidase in tobacco does not affect plant performance in controlled growth conditions[J] 2000,Photosynthesis Research(1):95～103
6Colin Robinson,Peter J. Hynds,David Robinson,Alexandra Mant. Multiple pathways for the targeting of thylakoid proteins in chloroplasts[J] 1998,Plant Molecular Biology(1-2):209～221
7J. Pospí?ilová. Satoh, K., Murata, N. (ed.): Stress Responses of Photosynthetic Organisms. Molecular Mechanisms and Molecular Regulations[J] 1998,Photosynthetica(3):360
8A. Kleinhofs,A. Kilian,M. A. Saghai Maroof,R. M. Biyashev,P. Hayes,F. Q. Chen,N. Lapitan,A. Fenwick,T. K. Blake,V. Kanazin,E. Ananiev,L. Dahleen,D. Kudrna,J. Bollinger,S. J. Knapp,B. Liu,M. Sorrells,M. Heun,J. D. Franckowiak,D. Hoffman,R. Skadsen,B. J. Steffenson. A molecular, isozyme and morphological map of the barley (Hordeum vulgare) genome[J] 1993,Theoretical and Applied Genetics(6):705～712
9Enrico Brugnoli,Olle Bj?rkman. Chloroplast movements in leaves: Influence on chlorophyll fluorescence and measurements of light-induced absorbance changes related to ΔpH and zeaxanthin formation[J] 1992,Photosynthesis Research(1):23～35

二级参考文献12

1Thayer S S,Bj?rkman O.Leaf xanthophyll content and composition in sun and shade determined by HPLC.Photosyn Res,1990,23:331～343
2Arvidsson P O,Bratt C E,Carlsson M,et al.Purification and identification of the violaxanthin deepoxidase as a 43 ku protein.Photosyn Res,1996,49:119～129
3Bugos R C,Yamamoto H Y.Molecular cloning of violaxanthin de-epoxidase from romaine lettuce and expression in Escherichia coli.Proc Natl Acad Sci USA,1996,93:6320～6325
4Bugos R C,Hieber A D,Yamamoto H Y.Xanthophyll cycle enzymes are members of the lipocalin family,the first identified from plants.J of Biol Chem,1998,273:15321～15324
5Niyogi K K.Photoprotection revisited:genetic and molecular approaches.Annu Rev Plant Physiol Plant Mol Biol,1999,50:333 ～ 359
6Demmig-Adams B,Adams W W Ⅲ.The role of xanthophyll cycle Carotenois in the protection of photosynthesis.Trends in Plant Science,1996,1:21～26
7Pfündel E,Bilger W.Regulation and possible function of violaxanthin cycle.Photosyn Res,1994,42:89～109
8Eskling M,Arvidsson P O,?kerlund H E.The xanthophyll cycle,its regulation and components.Physiol Plant,1997,100:806～816
9Müller P,Li X P,Niyogi K K.Non-photochemical quenching:a response to excess light energy.Plant Physiol,2001,125:1558～ 1566
10Lin R C,Ding Z S,Li L B,et al.A rapid and efficient DNA minipreparation suitable for screening transgenic plants.Plant Mol Biol Reptr,2001,19:379a～379e

共引文献4

1房蓓,王少峡.大麦紫黄素脱环氧化酶基因片段的克隆与分析[J].安徽大学学报（自然科学版）,2005,29(2):100-102.
2张旭强,季静,王罡,钟影,刁进进,关春峰,吴疆,金超.枸杞紫黄质脱环氧化酶(LcVDE)基因的克隆和分析[J].中国生物工程杂志,2014,34(1):21-27.
3张健,关婧雯,陈佳昌,李佳丽,孙丽娜,张宜欣,宋兴舜.渗透胁迫下vde基因缺失对拟南芥生理特性的影响[J].植物生理学报,2015,51(11):1975-1981.
4宋兴舜,孙丽娜,张秋艳,王瑞芳.VDE基因在植物抗逆响应中的功能[J].东北农业大学学报,2016,47(9):85-90.