AC-88对荷瘤小鼠抑瘤作用及对T细胞Fas蛋白表达的影响

The antitumor effect of AC-88 in mice bearing cancer and its effect on T cell Fas expression

目的 :探讨AC 88的抑瘤作用和可能机制。方法 :以移植H2 2肝癌细胞、艾氏腹水瘤细胞 (EAC)和MethA肉瘤细胞的荷瘤小鼠为体内实验模型 ,以培养的人大肠癌细胞株 (LOVO)、人卵巢癌细胞株 (TYK)和H2 2细胞为体外模型 ,研究AC 88对肿瘤细胞的抑制作用 ,并测定荷瘤小鼠的T细胞Fas蛋白的表达。结果 :给三种荷瘤小鼠灌服AC 882 5 0mg/kg组的体内抑瘤率分别高达 76 .31 %、73.5 2 %和 71 .91 % ,平均瘤重均低于对照组 (P <0 .0 1 ) ;体外的半数抑制浓度IC50 均小于 1 0μg/ml,而且AC 88能降低荷瘤小鼠的T细胞Fas蛋白的表达 (P <0 .0 1 )。 结论 :AC 88有良好的抑瘤作用 ,这种作用可能与AC

Objective:To investigate the antitumor effect and possible mechanism of AC 88.Methods:H22,Meth A,EAC cell bearing mice in vivo and LOVO,TYK,H22 culture cells in vitro were used to study the antitumor effect of AC 88 and Western blotting was used to study Fas expression of T lymphocytes.Results:The antitumor rate of AC 88 250 mg/kg group on three types tumor bearing mice was 76.31%,73.52%,and 71.91 %,respectively.The average tumor weight was lower than control group( P <0.01).The IC 50 of AC 88 on each type tumor cells was lower than 10 μg/ml in vitro .Fas expression of T cells was decreased in the same time( P <0.01).Conclusions:AC 88 has obvious antitumor effect and maybe through decrease T cell Fas expression.