红景天对心肌缺血再灌注损伤细胞因子影响的实验研究

Effect of Hongjingtian on Serumal Cytokines in Rat during Ischemia and Reperfusion Injury of Heart

目的 :利用SD大鼠心肌缺血再灌注 (I/R)模型 ,观察红景天对心肌缺血再灌注损伤时血中细胞因子水平的影响并探讨其作用机制。方法 :SD大鼠 40只 ,随机等分为 4组 :假手术组 (生理盐水 2ml/d)、I/R模型组 (生理盐水 2ml/d)、红景天组 [5 g/(kg·d) ]、苯那普利组 [10mg/(kg·d) ]。连续灌胃 5d后 ,各组均完成模型全部操作 ,A组只穿线不结扎冠状动脉 ,余组穿线并结扎冠状动脉 ,缺血、再灌注各 60min后处死。各组均取全血检测血中TNF -α、IL -6。结果 :红景天与模型组相比 ,血中TNF -α、IL -6明显降低(P <0 .0 1)。结论 :红景天对心肌缺血再灌注损伤有明显的保护作用。这种保护作用部分与其降低血细胞因子水平有关 ,从而为其临床应用提供理论依据。

Objective:To observe the effects of Hongjingtian on serumal cytokines in Rat during Ischemia and Reperfusion Injury of Heart and the possible action mechanism. Methods:40 male Spraegu-Dawley rats were randomly divided into four groups:sham operated group(A group, normal saline 2 ml/d), I/R group(B group, normal saline 2 ml/d), Hongjingtian-treated I/R group(C group,5g/kg/d) and benazepril-treated I/R group(D group, 10 mg/kg/d). After five days of pretreatment, the left anterior descending coronary arteries(LAD) of the ten rats in group A were false ligated.In group B,C,and D,the LADs were ligated and obturated for 60 minutes ,and then reperfused for 60 minutes. The whole bloods of all rats in each groups were collected and then centrifuged to determine the content of serumal TNF-α and IL-6.Results:The concentration of TNF-α and IL-6 were remarkably decreased and there was both obvious significance in the two treatment groups compared with model group.Conclusion:The results demonstrate that Hongjingtian provides similar remarkable protective effects in a rat model of ischemia reperfusion and the protective effects of Hongjingtian is partially due to reducing the concentration of TNF-α and IL-6.