壳聚糖在稀溶液中的聚集行为

Aggregation Behavior of Chitosan in Dilute Aqueous Solution

以 1 ,6 二苯基己三烯 (DPH)为探针分子 ,通过测定其在壳聚糖 (CS)水溶液中的荧光发射光谱及稳态荧光偏振值 ,研究了CS在稀溶液中的聚集行为。结果表明 ,壳聚糖在 0 1mol/L乙酸溶液中可明显自聚 ,当壳聚糖质量浓度达 1 0g/L时 ,溶液中DPH的荧光发射显著增强 ;十二烷基硫酸钠 (SDS)的加入显著地促进了壳聚糖在水溶液中的聚集 ;随着壳聚糖浓度的增加 ,壳聚糖分子链由舒展链结构转变为单链线团结构 ,进而转变为相互缠绕的线团结构。

The aggregation behavior of chitosan(CS) has been investigated with fluorescence probe technique. The fluorescence emission spectra and fluorescence polarization(p value) of 1,6-diphenyl hexatriene(DPH) in dilute aqueous solutions of CS were measured. Results indicate chitosan is able to self-aggregate and the aggregation could be obviously accelerated by the presence of sodium dodecylsulfate(SDS) in 0.1 mol/L acetic acid solution. The fluorescence emission of DPH was enhanced remarkably as the concentration of chitosan was 1.0 g/L. During the aggregation process, the structure of chitosan chains is transformed from stretched chains into coils and further transformed into intertwist coils.