高脂饮食胰岛素抵抗大鼠模型解偶联蛋白基因表达的变化

Change of Uncoupling Protein Gene mRNA Expresion in High-fat Induced Insulin Resistance Model

目的 研究高脂喂养致胰岛素抵抗 (IR)动物模型中胰岛素敏感性的变化及解偶联蛋白 (UCPs)基因表达的改变。方法 在高脂喂养大鼠的IR模型中 ,以高胰岛素 -正葡萄糖钳夹术评价胰岛素敏感性的变化 ,用半定量的逆转录 -聚合酶链反应 (RT -PCR)检测UCPs基因mRNA表达的改变。结果 高脂喂养可以显著降低葡萄糖输注率 (GIR2 8 0 3± 8 0 2VS 9 89± 3 2 8P <0 0 1) ,显著增加血清甘油三酯 (TG)、自由脂肪酸(FFA) (0 6 1± 0 17VS 1 2 4± 0 34,0 2 4± 0 10VS 0 4 3± 0 13) ,增加动物的体重 ,可使肝脏内UCP - 2基因、肌肉内UCP - 3基因mRNA表达降低 ,对肌肉组织内UCP - 2基因mRNA表达无影响。结论 高脂喂养可引起血脂增高、体重增加、降低胰岛素的敏感性 。

Objective To explore the change of insulin sensitivity and uncoupling protein gene(UCP) mRNA expression in high-fat rat model of insulin resistance. Methods The change of insulin sensitivity was valued by euglycomic-hyperinsulin clamp. The change of UCP mRNA in liver and muscle was measured using RT-PCR. Results Glucose disposal rate was reduced significantly (GIR 28.03±8.02 VS 9.89±3.28P<0.01),the level of serum triglycerides and FFA were increased (0 61±0 17VS1.24±0.34,0.24±0.10 VS 0.43±0.13),the body weight of animal also increased in high-fat group.Moreover, the expression of UCP-2 mRNA in liver and UCP-3 mRNA in muscle were lower in high-fat group, whereas UCP-2 mRNA level in muscle did not change insignificantly.Conclusion The high-fat diet can result in increasing of the level of serum lipid and the body weight, and reducing insulin sensitivity. The cause of insulin sensitivity reducing may be the lowering of UCPs Gene mRNA expression in high-fat induced insulin resistance model. KEY WORDS insulin resistance; insulin sensitivity; uncoupling protein