2型糖尿病患者血管并发症与血浆内皮素和一氧化氮的相关性及胰激肽释放酶干预治疗的研究

Study of the relationship of vascular complication with plasma endothelin and nitric oxide in type 2 diabetes and the effect of interfered treatment of TPK

目的 探讨 2型糖尿病患者血管并发症与血浆内皮素 (ET)、一氧化氮 (NO)的相关性及胰激肽释放酶 (TPK)干预治疗对其影响。 方法 随机选择 2型糖尿病患者 1 82例及正常对照 4 0名。放射免疫分析测定 (RIA)及酶连免疫吸附测定 (EL ISA)检测血浆 ET、NO水平和 TPK干预治疗后各指标的变化。 结果 2型糖尿病患者血浆 ET较正常人显著升高 (P<0 .0 1 ) ,伴血管并发症患者较单纯 2型糖尿病患者血浆 ET水平显著升高 (P<0 .0 1 )。 2型糖尿病血管并发症组较单纯 2型糖尿病组及正常对照组血浆 NO水平显著降低 (P<0 .0 1 )。2型糖尿病患者经 TPK干预治疗后血浆 ET水平降低 ,NO水平升高 ,尿白蛋白排泄率降低 ,血液流变学有所改善。 结论 内皮系统功能紊乱参与了 2型糖尿病血管并发症的发生。 TPK干预治疗可以改善 2型糖尿病患者内皮系统功能 ,防治糖尿病血管并发症的发生。

Objective To investigate the relationship of chronic diabetic vascular complication with plasma endothelin(ET) and nitric oxide (NO) level in type 2 diabetes and the effect of interfered treatment of TPK. Methods 182 cases of type 2 diabetes and 40 healthy controls were recruited into the study. Plasma ET and NO level were measured by RIA and ELISA. The measurements were also carried out before and after the interfered treatment of TPK. Results The plasma ET level was significantly higher in diabetes than that in healthy controls ( P <0.01). The plasma ET level of diabetes with vascular complication was higher than that of diabetes without vascular complication ( P <0.01). The plasma NO level was significantly lower in diabetes with vascular complication than that in diabetes without vascular complication and healthy controls ( P <0.01). But there was no difference in plasma NO level between healthy controls and diabetes patients without vascular complication( P >0.05). The levels of plasma NO increased and levels of plasma ET decreased in diabetes after the treatment of TPK. Conclusions Endothelial dysfunction is an essential factor in the occurrence and development of diabetic vascular complication. The interfered treatment of TPK may prevent the development of type 2 diabetes and its vascular complication.