CIK治疗后肿瘤患者外周血T淋巴细胞亚群和NK细胞变化研究

Study on Change of T Cell Subsets and NK Cells in Peripheral Blood from Patients with Tumor Treated with CIK

目的:探讨CIK(CellInductionKiller)治疗后肿瘤患者的免疫功能状态。方法:采用流式细胞术检测20例肿瘤患者外周血T淋巴细胞亚群和NK细胞。结果:肿瘤患者CD3+、CD4+、CD3-/CD16+56+细胞和CD4/CD8比值明显低于对照组并呈显著性差异,而CD8+细胞高于对照组(P<0.01)。CIK生物治疗后CD3+、CD4+、CD3-/CD16+56+细胞和CD4/CD8比值都升高,其中CD4+、CD3-/CD16+56+细胞与治疗前比较呈显著性差异(P<0.05),而CD8+细胞与治疗前比较降低(P<0.05)。结论:肿瘤患者的免疫功能低下,CIK生物治疗能够提高机体的免疫功能。流式细胞术对患者免疫功能的监测,具有快速、灵敏、准确的特点,对于评价疗效、判断预后具有重要价值。

Objective:To investigate the immunological function of tumor patients treated with CIK.Methods:The T cell subsets and NK cells in peripheral blood from patients with tu-mor(n=20)were detected with flow cytometry.Results:The CD3 + 、CD4 + 、CD3 - /CD16+56 + cells and CD4/CD8from patients with tumor are significantly lower than that of the control(P<0.01).Com paried with the control group,the CD 8+ cells from patients with tumor were increased(P<0.01).The CD3 + 、CD4 + 、CD3 - /CD16+56 + cells and CD4/CD8from patients treated with CIK were in-creased.Comparied with pre-treatment,the difference of CD4 + 、CD3 - /CD16+56 + cells is significant (P<0.05)and the CD8 + cells were decreased(P<0.05).Conclusions:The immunological function of patients with tumor were decreased and could be enhanced by CIK biotherapy.The flow cytome -try is a rapid、sensitive and accurate method to detect the immunological function of patients with tumor.It can play an important role to value curative effect and judge prognosis of the diseases.