明胶酶A在急性白血病细胞中的表达及其临床意义

The expression and clinical implication of gelatinase A in acute leukemic cells

目的 探讨急性白血病 (AL)细胞中明胶酶A(MMP2 )的表达及其临床意义。方法 应用明胶酶谱法检测了 4 6例初治AL患者MMP2的表达 ,同时应用逆转录 PCR(RT PCR)测定了MMP2活化相关基因基质金属蛋白酶抑制剂 2 (TIMP2 )、基质金属蛋白酶 1(MT1 MMP)的表达 ,并在体外进行了白血病细胞株穿膜试验研究。结果 4 6例AL患者中 2 4例 (5 2 2 % )MMP2表达阳性 ,MMP2阳性组与阴性组发生髓外浸润的比例分别为 5 0 0 %和 18 2 % (P值均 <0 0 5 ) ;外周血幼稚细胞百分率分别为 (77 2 1± 13 9) %和 (6 2 95± 17 2 ) % (P值均 <0 0 1)。同时 4 6例AL患者中TIMP2、MT1 MMP阳性表达分别为 2 7例 (5 8 7% )、33例 (71 7% )。体外穿膜试验证实了MMP2对白血病细胞侵袭能力的影响。

Objective To study the expression of gelatinase A (MMP2) in acute leukemic cells and its clinical implication Methods The bone marrow samples from 46 acute leukemia patients were investigated by reverse transcription polymerase chain reaction (RT PCR) and Gelatin Zymography method Matrigel invasion assay was studied on U937, NB4, K562 and SHI1 cells in vitro .Results The expression of MMP2 was positive in 24 of 46 patients with AL(52 2%). In MMP2 positive and negative groups of AL, the extramedullary infiltration rates were 50.0% and 18 2%( P <0 05),whilst the percentage of blast cell in peripheral white blood cells were (77 21±13 9)% and (62 95±17 2)%( P <0 01), respectively The positive expression of TIMP2 and MT1 MMP in 46 AL patients were 27 (58 7%)and 33(71 7)% The matrigel invasion assay suggested that MMP2 is involved in migration of leukemic cell through extracellular matrix (Matrigel) Conclusion The active form of MMP2 might be essential to the egress of leukemic cells from bone marrow into peripheral blood, followed by an extramedullary infiltration