摘要
目的 探讨前炎细胞因子对鼻息肉上皮细胞表达人类白细胞抗原 (humanleucocyteantigen ,HLA) DR的影响。方法 2 3例鼻息肉及 1 1例鼾症患者下鼻甲标本 ,固定包埋 ,以HLA DR单抗免疫组化染色以观察HLA DR阳性细胞的分布。另分离其上皮细胞体外培养 ,并以白介素 1 β(interleukin 1 β ,IL 1 β) ,肿瘤坏死因子 α(tumornecrosisfactor,TNF α) ,IL 1 β +TNF α ,IL 1 β +TNF α +地塞米松分别刺激 ,采用免疫组化SP法 ,检测HLA DR在鼻息肉、下鼻甲组织上皮细胞及炎性因子下培养上皮细胞中的表达及分布。结果 HLA DR在鼻息肉组织中主要表达于上皮细胞层。在鼻眶手术患者下鼻甲组织中上皮表面纤毛层有轻微表达。培养的鼻黏膜上皮细胞阳性表达在胞浆 ,发现 :①对照组阳性细胞表达极少 ,鼻息肉组与下鼻甲组差异无显著性 ;②加入IL 1 β或TNF α ,HLA DR表达明显增加 ,与对照组差异显著 ,IL 1 β+TNF α刺激组表达最强 ,且鼻息肉上皮细胞表达强于下鼻甲组 ;③加入地塞米松后 ,各组表达水平下降 ,鼻息肉组与下鼻甲组无明显差别。结论 鼻息肉上皮细胞在前炎细胞因子作用下通过HLA DR分子的表达参与抗原提呈和炎症过程。糖皮质激素通过下调HLA DR的表达 ,可作为免疫抑制剂减轻局部免疫损伤。
Objective To explore the effect of proinflammatory cytokines on the expression of human leucocyte antigen(HLA DR) in epithelial cell of nasal polyps Methods The samples were from 23 patients with nasal polyps and 11 cases of normal inferior turbinates The samples were fixed,paraffin embeded and sectioned for observing distribution of HLA DR positive cells Nasal epithelial cells were obtained after enzymatic digestion and cell culture was performed for observing influences of interleukin(IL) 1β and tumor necrosis factor (TNF α) to expression of HLA DR of epithelial cells and the effect of dexamethasone(10 -5 mol/L) Results The HLA DR expression in epithelium was significantly stronger in nasal polyps than that in inferior turbinates The expression of HLA DR in cultured cells under stimulation of proinflammatory cytokines was increased compared with controls, but the expression was more intense in nasal polyps than that in inferior turbinates Increased HLA DR expression under stimulation of cytokines was reduced significantly by dexamethasone Conclusion The epithelium is in a key position to participate in inflammatory and immunologic events in the airways Epithelial cells potentially contribute to airway inflammation by antigen presentation and the production of proinflammatory cytokines
出处
《中华耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2003年第3期183-186,T003,共5页
Chinese Journal of Otorhinolaryngology
