氟致雄性大鼠生殖功能损害的实验研究

Experimental study on effect of fluoride on reproductive system of male rats

目的 探讨高氟对雄性大鼠生殖系统的影响。方法 给予雄性 Wister大鼠含氟化钠 15 0 m g/ L 的水 10周后 ,检测其精子计数、活动率、畸形率 ,测定睾丸、附睾生化标志酶和性激素 ,观察睾丸组织病理改变。结果 染氟组精子计数、活动率下降 ,畸形率升高 ,血清睾酮、促黄体生成激素下降 ,与对照组比较差异有统计学意义(P <0 .0 5 ) ,睾丸组织有明显的病理学改变。结论 长期接触高浓度的氟对雄性生殖系统有毒性作用。

Objective To study the effects of excessive fluorine on reproductive system of male rats.Methods Sodium fluoride was administered to male rats with drinking water (150 mg/L) for 10 weeks. The sperm count, the rates of sperm mobility and aberration, the levels of biochemical marker enzyme of testis and epididymidis, as well as the levels of gonadal hormone in serum, were examined. And the pathological alterations of testes were observed.Results Contrasted with control rats, the sperm count, the rate of sperm mobility, as well as the levels of serum testosterone (T) and luteinizing hormone (LH), were suppressed by fluoride. While the rate of sperm aberration was elevated. The differences between two groups were significant ( P <0.05). The pathological alterations of testes were observed.Conclusions The long-term exposure to fluorine of high dose could produce toxicity effect on male reproductive system.