14种微藻总脂含量和脂肪酸组成研究

TOTAL LIPID AND FATTY ACID COMPOSITION OF 14 SPECIES OF MIRCOALGAE

比较分析了 1 4种微藻的总脂含量和脂肪酸组成 ,结果表明 :除小球藻、亚心形扁藻、极微小环藻、微绿球藻外 ,其他微藻的总脂含量均超过其干重的 1 0 %。每一纲的微藻脂肪酸组成都有各自特点 ,绿藻纲中 1 6∶0、1 6∶1(n - 7)、1 8∶1 (n - 9)含量较高 ,但微绿球藻中 1 6∶1 (n- 7)、2 0∶5(n - 3) (EPA)含量远高于其他绿藻 ;金藻纲中含大量1 4∶0、1 6∶0、1 8∶3(n - 3)、2 2∶6(n - 3) (DHA) ;硅藻纲中 1 4∶0、1 6∶0、1 6∶1 (n - 7)、EPA含量较高 ;黄藻纲的异胶藻富含1 6∶0、1 6∶1 (n - 7)和EPA。

species of microalga at exponential phase of growth be cultured in MAV substrate in the condition of water tempreture 20±1℃,salinity 34 and nature illumination. The microalga be collected by 4000～8000r/min centrifuge 10 minutes after 7 day's culute, the deposition has been rinsed with asepsis water 3 times be lyophilizated and then be freeze preserved(-4℃). The dry alga glomerules be homogenated with bead mill homogenizer for assay of total lipid and every fatty acid content each by chloroform methanol tecknique and gas chromatography. The assay results showed that the total lipid contents of the microalgae were more than 10% on dry weight except 4 species. Each class of microalgae was characterized by a specific fatty acid profile. The specific feature of Chlorophyceae were high concents of 16∶0,16∶1 (n 7) ,and 18∶1(n 9)except N. oculatas which contain a little 16∶3(n 3) and no 18∶3(n 3),but contained high cocentration of 20∶5(n 3). The specific features of Chrysophyceae were 14∶0,16∶0,18∶3(n 3)and 22∶6(n 3). The specific feature of Bacillariophyceae were high in contents of 14∶0,16∶0,16∶1(n 7) and 20∶5(n 3). The specific feature of Heterogloea sp. were high in contents of 16∶0,16∶1(n 7) and 20∶5 (n 3) .