发育中的人胎小肠神经肽的研究

Study on Development and Distribution of Neuropeptides in Human Fetus Small Intestine

目的 研究人胎小肠壁P物质 (SP)和降钙素基因相关肽 (CGRP) IR肽能神经的分布和发育规律。方法 苏木精伊红 (HE)染色和免疫组织化学ABC法。结果 在胎儿发育过程中 ,小肠组织形态及其神经肽的发生、分化出现明显的变化 ,其分化由十二指肠向空肠、回肠依次进行。第 14周开始 ,胎儿小肠壁粘膜下层、肌层间结缔组织中偶见SP能、CGRP能神经纤维及神经元免疫反应产物 ,第 34周至 38周时反应最强 ,神经元从浅棕色到深棕色 ,神经纤维呈串珠状或点线状。从SP、CGRP免疫组织化学相邻切片上看 ,两者部分存在共存现象。结论 人胎小肠壁存在SP IR、CGRP IR肽能神经 ,粘膜下和肌间神经丛存在SP IR、CGRP IR肽能神经元和神经纤维 。

Objective To study the distribution and developmental regularities of Substance P(SP) and Calcitonin Gene Related Peptide(CGRP) IR peptidergic nerves in fetus small intestine. Methods Hematoxylin Eosin(HE) staining and immunohistochemical ABC method were used.Results The histologic form of small intestine and its neuropeptides appeared obvious changes in developing fetus, its differentiation proceeded successively from duodenum to jejunum and ileum. At 14th week, the SP IR and CGRP IR nerve fibers and cells appeared occasionally in the myenteric and submucous plexuses in duodenum, and the responds were the most strong in the period of 34th week to 38th week. Neurons were stained from light to deep brown, and nerve fibers were presented as varicose and liner profiles. On the corresponding site of serial sections of SP IR?CGRP IR immunohistochemistry, some of them coexisted in one nerve fiber and cell.Conclusion The SP IR and CGRP IR peptidergic nerves are seen in fetus small intestine. The SP IR and CGRP IR nerve fibers and cells appear in the myenteric and submucous plexuses, but their development and distribution are different.