p27基因及其蛋白在大肠癌的表达及其意义

Expression and significance of p27 mRNA and protein in colorectal carci noma

目的 研究 p2 7mRNA和p2 7蛋白在大肠癌的表达及其临床意义。方法 应用原位杂交方法检测 58例大肠癌标本及 58例正常黏膜中p2 7mRNA的表达 ,应用免疫组织化学S P法检测相同组织中 p2 7蛋白的表达。 结果 p2 7mRNA在大肠癌及正常黏膜中的表达阳性率均为 1 0 0 %。p2 7蛋白在大肠癌中的表达阳性率为 55 1 7% (32 / 58) ,在正常组织中的表达阳性率为 96 55 % (56/ 58) ,差异有显著性(P <0 0 1 )。p2 7蛋白表达与肿瘤组织分化程度负相关 ,与其它临床病理因素无关。结论 p2 7蛋白表达的调控主要在转录后水平。p2 7蛋白表达在大肠癌发生过程中具有重要作用 。

Objective To investigate the expression and clinica l significance of p27 mRNA and protein in colorectal carcinoma. Methods In situ hybridization for p27 mRNA was performed in 58 colorectal carcinoma an d 58 normal mucosa. Immunohistochemical staining (S-P method) for p27 protein w as performed in the same cases. Results The positive rates of p27 mRN A expression in colorectal carcinoma and normal colorectal mucosa were both 100%. Significant difference of p27 protein expression was found between normal mucosa and carcin omas (P<0 01). p27 protein expression was inversely correlated with histol ogical grade (P<0 01). No relationships were found between p27 protein and other clincopathological characteristics (P>0 05). Conclusion T he expression of p27 mRNA is not correlated to the expression of p27 protein. p2 7 protein expression is regulated mainly at posttranscriptional level. p27 prote in plays an important role in carcinogenesis of colorectal carcinoma. Protein ex amination of p27 assists in evaluating the malignant degree and prognosis of col orectal carcinoma.