胆道支撑管显影材料的制备及体外细胞毒性评价

Preparation and cytotoxicity evaluation in vivo of new-type radiopacity materials for biliary supporting tube

目的 探讨新型显影胆道支撑管材料制备的可行性 ,对构建出的材料进行体外细胞毒性评价和筛选。方法 选择具有X线下显影特性的基础材料A、B、C ,分别与硅橡胶生胶共混炼 ,模压后分别得到相应的显影复合材料甲、乙、丙 ,经一段和二段硫化过程完成加工。采用MTT法作为体外细胞毒性评价的方法 ,测定参比对照材料和新材料的 5 0 %浓度的浸提液对小鼠成纤维细胞的增殖率的影响 ,确定细胞毒性级别。结果 新材料甲、乙、丙在X线下影像清晰。甲、乙材料的细胞毒性级别为零 ,丙材料细胞毒性级别为 2。结论 构建出在X线下显影的弹性体材料可行。材料甲、乙是制造新型胆道支撑管的初选材料 ,材料丙无应用的价值。

Objective To explore the possibility of preparing non radioactive materials for manufacturing new type biliary supporting tubes, evaluate the cytotoxicity of the materials according to the standards of ISO and screen a suitable one for further researches. Methods Three basic non radioactive materials A0, B0 and C0 were mixed with the raw silastic material in accordance with a weight ratio. Then the compounds were melted, molded and placed for two phase vulcanization to obtain relevant new radiopacity silicone elastoers A, B and C. MTT was adapted to examine the cytotoxicity in vivo of these 3 new materials. Results All the 3 new materials showed satisfactory images in the X ray examination. The toxicity grade was grade II for sample C and zero for sample A and sample B. Conclusions New materials with a satisfactory radiopacity efficacy can possibly be prepared now. Materials A and B, which possess both radiopacity and compatible features, are initially chosen for development of the biliary supporting tubes. Materials C with cytotoxicity grade Ⅱ should be rejected in the further study.