供者凋亡细胞预输注诱导同种器官移植免疫抑制的研究

Immumosuppression of allotransplantation induced by transfusion of donor apoptotic cells

目的 探讨凋亡细胞对受者器官移植免疫系统的作用。方法 应用紫外线为细胞凋亡诱导工具 ,以大鼠心脏移植作为动物模型。将紫外线照射后培养 8h的供者凋亡脾细胞注入受鼠体内 ,分别在输注后 0、3、7、14d将供鼠心脏移植至受鼠腹腔内 ,观察移植物存活时间。结果 紫外线能有效诱导大鼠脾细胞凋亡 ;预输注供者凋亡脾细胞组移植心脏存活期明显延长 ,以输注 7d后移植者最为显著 ,而输注同种活细胞或死细胞组则无此效应。结论 凋亡细胞能主动抑制受者淋巴细胞活性 ,延长同种移植物存活时间 ,利用凋亡细胞诱导器官移植免疫抑制是一条可行的途径。

Objective To investigate the influence of apoptotic cells on organ transplantation.Methods Apoptosis was induced by ultraviolet irradiation and detected by flowcytometry.Ultraviolet irradiated donor spleen cells were injected to recipients prior to rat heterotopic heart transplantation at day 0,3,7 and 14.The allograft survival time was measured.Results Ultraviolet irradiation could induce apoptosis effectively.In the allogeneic Wistar-to-SD combination,preoperative intravenous injection of ultraviolet irradiated donor spleen cells resulted in a significant prolonged graft survival as compared with control.The best transfusion time was at 7th day (45.13±49.43 vs 7.33±0.87),while administration of normal or necrosis spleen cells had no such effect (8.00±1.41,9.75±1.55 respectively).Conclusion Preoperative administration of donor apoptotic cells prolonged graft survival.It is probably a feasible method to induce organ transplantation immune tolerance by donor apoptotic cells.